DNA image cytometry in breast fine needle aspiration cytology

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Author:
WANG Rui(Center of Cancer Detection,Fourth Hospital of Hebei Medical Univesity,Shijiazhuang 050011,China)
WANG Heng(Center of Cancer Detection,Fourth Hospital of Hebei Medical Univesity,Shijiazhuang 050011,China)
DU Yun(Center of Cancer Detection,Fourth Hospital of Hebei Medical Univesity,Shijiazhuang 050011,China)
Journal Title:
Cancer Research and Clinic
Issue:
Volume 24, Issue 08, 2012
DOI:
10.3760/cma.j.issn.1006-9801.2012.08.006
Key Word:
DNA; Image cytometry; Breast masses; Fine meedle aspiration cytology

Abstract: Objective To discuss the diagnosis value of DNA imaging cytometry (DNA-ICM) analysis in fine needle aspiration cytology (FNAC).Methods Conventional cytopathology and DNA-ICM were used to detect 95 fine needle aspiration patients with breast masses.The results were analyzed according to the histopathological diagnosis.Results There was not significant difference between conventional cytopathology and DNA-ICM [88.4 %(84/95) vs.94.7 %(90/95)] (x2 =2.457,P =0.117).Two false positive cases had been found in conventional cytopathologic diagnosis,but not in DNA-ICM.There was a positive correlation between the nucleus area and its DNA content (r =0.744,P =0.000).By DNA-ICM,the detectable rate of abnormal DNA content in the infiltrating ductal carcinoma was higher than that in the infiltrating lobular carcinoma [90.3 %(65/72) vs.50.0 %(4/8)] (P =0.011).Conclusion The detection of DNA-ICM can reduce the false positive rate of FNAC,but it has limitations in diploid tumors.

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