Effect of blocking the signal pathway of TGFβ on presenting antigen of dendritic cells
- Author:
- No author available
- Journal Title:
- Cancer Research and Clinic
- Issue:
- 10
- DOI:
- 10.3760/cma.j.issn.1006-9801.2011.10.002
- Key Word:
- 树突细胞;转化生长因子β;腺病毒载体;Smad7;Dendritic cells;Transforming growth factor beta;Adenoviral vector;Smad7
Abstract： Objective To explore the dysfunction of dendritic cells (DC) related to TGFβ reversed after blocking the TGFβ signal pathway by recombinant adenovirus vector encoding for Smad7.Methods Smad7 by recombinant adenovirus vector was transfected into dendritic cells.Expression of immunologic phenotypes was detected by FCM,and CTL activity induced by DC was compared.Results The DC modified with Smad7 still expressed high adhesiveness factor related to maturation even if existing exogenous TGFβ1,which was significant statistically compared with DC transfected with control adenoviral vector (P ＜0.01).Even if existing exogenous TGFβ1,the DC modified with Smad7 pulsed with soluble antigen associated with Lewis pulmonary carcinoma could still induce potent CTL activity against Lewis pulmonary carcinoma,which showed significant difference with DC-Ad-c (P ＜0.01).Conclusion The inhibitory effects on function of DC of TGFβ may be reversed by blocking the Smad signal of TGFβ pathway.
- [1]秦建军,周清华,覃扬,等.Smad3D和Smad7重组腺病毒载体的快速构建[J].2003,(3).
- [2]谢艳云,樊卫平,李娜,等.超声抗原树突状细胞瘤苗抗肿瘤效应的体外研究[J].2010,(10).
- [3]Whitman, Malcolm.Feedback from inhibitory SMADs.[J].1997,389(6651).
- [4]Ortegel JW,Staren ED,Warren WH,etc.Modulation of tumor-infiltrating lymphocyte cytolytic activity against human non-small cell lung cancer.[J].2002,36(1).
- [5]K, Inaba,M, Inaba,N, Romani,etc.Generation of large numbers of dendritic cells from mouse bone marrow cultures supplemented with granulocyte/macrophage colony-stimulating factor.[J].1992,176.
- [6]Albert Lin,Anita Schildknecht,Linh T. Nguyen,etc.Dendritic cells integrate signals from the tumor microenvironment to modulate immunity and tumor growth[J].2010,127(2).
- [7]Angus W. Thomson,Lina Lu,Noriko Murase,etc.Microchimerism, dendritic cell progenitors and transplantation tolerance[J].1995,13(6).
- [8]Yigit R,Massuger LF,Figdor CG,etc.Ovarian cancer creates a suppressive microenvironment to escape immune elimination.[J].2010,117(2).
- [9]Deane NG,Halder SK,Rachakonda G.Smad7 induces hepatic metastasis in colorectal cancer.[J].2008,99(6).