Abstract： Objective To observe and detect the change of invasion and migration about CNE cell line after 5-ALA Photodynamic therapy. Methods The relation between the inhibition rate and 5-ALA concentration & energy density of lasers was detected by MTT. Scratch wound assay and matrigel artificial basement membrane invasion assay were perforned after photodynamic therapy with different photosensitizer concentration and energy density of lasers. Results The results showed that photodynamic therapy can inhibit the growth of CNE cells. The migration of CNE cell was inhibited with appropriate photosensitizer concentration and energy density of laser after photodynamic therapy. It exhibited a linear relation between cell migration and the energy density, when the photosensitizer concentration was a maximum effective concentration of 1 mmol/L (P ＜0.05). The invasion of CNE cell was also inhibited after photodynamic therapy,there is a relationship between the change of invasion and 5-ALA concentration & energy density of lasers. It which exhibited a linear relation between cell invasion and the energy density in the same photosensitizer concentration (r ＞0.8, P ＜0.0001). Conclusion The migration and invasion of CNE cells are both inhibited after photodynamic therapy. The change of migration and invasion is related to 5-ALA concentration & energy density of laser.