A relationship between genetic polymorphisms of CYP2E1 and susceptibility of acute leukemia in Gansu population

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Author:
SHI Xiu-e(Department of Gerontology, Rehabilitative Central Hospital of Gansu, Lanzhou 730000,China)
XU Jian-wang()
MA Jia-ling(Department of Gerontology, Rehabilitative Central Hospital of Gansu, Lanzhou 730000,China)
LIU Hai(Department of Gerontology, Rehabilitative Central Hospital of Gansu, Lanzhou 730000,China)
JIA Ming-feng()
LIU Chun-xia()
XI Ya-ming()
Journal Title:
CANCER RESEARCH AND CLINIC
Issue:
Volume 22, Issue z1, 2010
DOI:
10.3760/cma.j.issn.1006-9801.2010.增.002
Key Word:
Cytochrome P450 CYP2E1;Genetic polymorphism;Susceptibility;Acute leukemia

Abstract: Objective To study the relationship between genetic polymorphisms of CYP2E1 and the susceptibility of the acute leukemia in Gansu population. Methods The C609T polymorphism of CYP2E1 gene was detected by polymerase chain reaction-ligase detection reaction (PCR-LDR) and 1∶1 matched casecontrol method in 100 healthy persons (control group) and 100 patients with acute leukemia (AL group).Results The C2 allele genotype and C1C2/C2C2 genotype of CYP2E1 gene occurred more frequently in AL group (13.5 % and 22 %, respectively) than those in control group (10.5 % and 19 %, respectively), however,both differences showed no statistical significant. Further stratified analysis, the C1C2/C2C2 genotype of CYP2E1 gene occurred more frequently in AML group (27%) than that in control group (19 %), but difference had no statistical significant, too. The occurrence frequency of the C2 allele genotype and C1C2/C2C2 genotype of CYP2E1 gene showed no significant difference in ALL group and control group (x2=0.446, P =0.504>0.05). Conclusion Genetic polymorphisms of CYP2E1 don't correlated to susceptibility of acute leukemia(AML and ALL) in Gansu population.

  • [1]Seaton MJ,Follansbee MH,Bond JA.Oxidation of 1,2-epnxy-3-butene to 1,2∶3,4-diepoxybutane by cDNA-expressed human cytochromes P450 2E1 and 3A4 and human,mouse and rat liver microsomes.Carcinogenesis,1995,16:2287-2294.
  • [2]Umeno M,Mcbride OW,Yang CS,et al.Human ethanol-inducible P4502E1:complete gene sequence promoter characterization,chromosome mapping,and cDNA directed expression.Biochemistry,1988,27:9006-9013.
  • [3]Watanabe J,Hayashi SI,Nakachi K,et al.PstI and RsaI RFLPs in complete linkage disequilibrium at the CYP2E gene.Nucleic Acids Res,1990,18:7194-7204.
  • [4]Hayashi S,Watanabe J,Kawajiri K.Genetic polymorphisms in the 5'-flanking region change transcriptional regulation of the human cytochrome P450IIE1 gene.J Biochem,1991,110:559-565.
  • [5]Daly Ak,Fairbrother Ks,Smart J.Recent advances in understanding the molecular basis of polymorphisms in genes encoding cytochrome P450 enzymes.Toxicol Lett,1998,102:143-147.
  • [6]Watanabe J,Hayashi S,Kawajiri K.Different regulation and expression of the human CYP2E1 gene due to the Rsal polymorphism in the 5'-flanking region.J Biochem,1994,116:321-326.
  • [7]Fairbrother Ks,Grove J,De Waziers I,et al.Detection and characterization of novel polymorphisms in the CYP2E1 gene.Pharmacogenetics,1998,8:543-552.
  • [8]Carte S,Crosti F.A nomenclature system for metabolic gene polymorphisms.Lyon:IARC scientific publications.1999,148:5-12.
  • [9]Nagai F,Hiyoshi Y,Sugimachi K,et al.Cytochrome P450 (CYP)expression in human myeloblastic and lymphoid cell lines.Biol Pharm Bull,2002,25:383-385.
  • [10]Canalle R,Burim RV,Tone LG,et al.Genetic polymorphisms and susceptibility to childhood acute lymphoblastic leukemia.Environ Mol Mutagen.2004,43:100-109.
  • [11]Krajinovic M,Sinnett H,Richer C,et al.Role of NQO1,MPO and CYP2E1 genetic polymorphisms in the susceptibility to childhood acute lymphoblastic leukemia.Int J Cancer,2002,97:230-236.
  • [12]吴玉霞,高怡瑾,赵金彩.GSTM1、CYP2E1和NQO1基因多态性与儿童白血病发病风险的初步研究.中华流行病学杂志,2004,25:819.
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