Study on the anti-tumor effects of the sonicate sensitized DC vaccine in vitro
- Author:
- No author available
- Journal Title:
- CANCER RESEARCH AND CLINIC
- Issue:
- 10
- DOI:
- 10.3760/cma.j.issn.1006-9801.2010.10.005
- Key Word:
- 肿瘤;癌症疫苗;树突细胞;T淋巴细胞,细胞毒性;免疫;SW480;Neoplasms;Cancer vaccines;Dendritic cell;T-lymphocyte,cytotoxic;Immunologic;SW480
Abstract： Objective To explore the cytotoxicity of the cytotoxic T lymphocyte (CTL) induced by SW480 sonicate sensitized dendritic cells (DC) on the colon cancer cell line SW480. Methods PBMC were separated from the HLA-A*0201 donor and DC were cultured with rhGM-CSF, rhIL-4 and rhTNF-α. The same donor's primary CTL were stimulated by DC loaded with SW480 sonicate. The cytotoxicity of CTL on SW480 (HLA-A*0201 positive) and K562 (HLA-A*0201 negative) was determined by the MTT method. Results The cytotoxicity of the CTL on SW480 was stronger than that on K562 (P ＜0.05). Conclusion The DC vaccine can stimulate specific CTL which can trigger cytotoxic activity on the target cells and this cytotoxicity is related to MHC restriction.
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