Abstract： Objective To investigate the expression of CA242 and CD_(44v6) protein in gastric cancer (GC) and its clinical significance. Methods Immunohistochemical staining was used to detect the expression of CA242 and CD_(44v6) protein in 120 cases of GC, 30 cases of gastric epithelial dysplasia and 20 cases of normal gastric mucosa. The pathological feature of GC and clinical follow-up data was also analysed. Results The positive rate of CA242 and CD_(44v6) was 79.2 % and 81.7 %, respectively in primary GC. The positive rate of CA242 and CD_(44v6) in GC was higher than that in epithelial dysplasia and normal gastric mucosa (P <0.05). The expression of CA242 and CD_(44v6) was closely related to infiltration of serosa, metastasis of lymph node and prognosis of patients (P <0.05). Conclusion These data suggest that the expression of CA242 and CD_(44v6) is closely related to carcinogenesis, metastasis and survival rate in GC. CA242 and CD_(44v6) may be prognostic indicators of GC.