The tyrosine phosphatase SHP-2 participated in the cell migration induced by IL-1β
- Author:
- No author available
- Journal Title:
- CANCER RESEARCH AND CLINIC
- Issue:
- 12
- DOI:
- 10.3760/cma.j.issn.1006-9801.2008.12.002
- Key Word:
- 蛋白酪氨酸磷酸酶;钙黏着糖蛋白类;基质金属蛋白酶9;白细胞介素1β;Protein-tyrosine phosphatase;Cadherin;Matrix metalloproteinase 9;Interleukin-1 beta
Abstract： Objective To investigate whether the tyrosine phosphatase SHP-2 participate in the cell migration induced by IL-1β.Methods Two different reorganized plasmids transfected into the MCF-7,and achieved two different cell lines:SHP-2-GFP-MCF-7 cell lines.SHP-2C＞S-GFP-MCF-7 cell lines.The cell migration was detected by a fluorescence microscope and the expression of E-eadhefin and MMP-9 wee detected by western blotting analysis and RT-PCR.Results The SHP-2-GFP-MCF-7 cell lines showed a higher cell migration ability,and the expression of E-cadherin in the SHP-2-GFP-MCF-7 cell lines was especially lower,neady undetectable.But in this group the expression of MMP-9 was the highest.Conclusion The tyrosine phosphatase SHP-2 participate in the cell migration induced by IL-1β,and maybe produce a marked effect by reinforcing the expression of MMP-9 and reducing the expression of E-cadherin.
- [1]Henegariu O,Qu CK,Feng GS,etc.Protein-tyrosine phosphatase Shp-2 regulates cell spreading, migration, and focal adhesion.[J].1998,273(33).
- [2]Tsuda M,Inagaki K,Noguchi T,etc.Roles for the protein tyrosine phosphatase SHP-2 in cytoskeletal organization, cell adhesion and cell migration revealed by overexpression of a dominant negative mutant.[J].2000,19(1).
- [3]Aaronson SA,Phillips GR,Norton L,etc.Exogenous expression of N-cadherin in breast cancer cells induces cell migration, invasion, and metastasis.[J].2000,148(4).
- [4]Mossink MH,Bennett AM,Zito CI,etc.The major vault protein is a novel substrate for the tyrosine phosphatase SHP-2 and scaffold protein in epidermal growth factor signaling.[J].2004,279(28).
- [5]Herrera Abreu MT,Chow CW,Vachon E,etc.The protein tyrosine phosphatase SHP-2 regulates interleukin-1-induced ERK activation in fibroblasts.[J].2003,278(29).
- [6]Kim SH,Li Z,Sacks DB.E-cadherin-mediated cell-cell attachment activates Cdc42.[J].2000,275(47).
- [7]S, Ahmad,D, Banville,Z, Zhao,etc.A widely expressed human protein-tyrosine phosphatase containing src homology 2 domains.[J].1993,90(6).