Effects of thalidomide on proliferation of human renal cancer cell and expression of basic fibroblast growth factor
- Author:
- No author available
- Journal Title:
- CANCER RESEARCH AND CLINIC
- Issue:
- 11
- DOI:
- 10.3760/cma.j.issn.1006-9801.2008.11.002
- Key Word:
- 细胞系,肿瘤;成纤维细胞生长因子;肾肿瘤;沙利度胺;Cell lines,tumor;Fibroblast growth factors;Renal neoplasms;Thalidomide
Abstract： Objective To elucidate the effects of thalidomide on proliferation of human renal cell line 786-0 and the expression of basic fibroblast growth factor (bFGF) in this cell line. Methods Cell was treated with different doses of thalidomide(6.25 μg/ml, 25 μg/ml, 100 μg/ml) respectively or normal saline as control; cell survival rate was analyzed by MTT assay. The mRNA level of bFGF was detected by reverse transcriptase-polymerase chain reaction (RT-PCR). bFGF protein expression in 786-0 cells was detected by flow cytometry (FCM). Results Thalidomide, ranging from 6.25 μg/ml to 100 μg/ml, suppressed the proliferation of 786-0 cell line in vitro significantly. After application of thalidomide for 48 and 72 hours, the IC50 was 46.42 μg/ml and 19.56 μg/ml respectively. Apoptosis rate increased from 12.43 % to 30.30 %, accompanying with reducing expression of bFGF. Application of thalidomide (25 μg/ml) induced the most significant inhibition to the bFGF in the cell line. Conclusion Thalidomide down-regulates bFGF expression, inhibit the proliferation, and induce apoptosis in 786-0 cell line.
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