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Establishment of lymphatic metastasis model for human gastric cancer in nude mice
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- Author:
- No author available
- Journal Title:
- CANCER RESEARCH AND CLINIC
- Issue:
- 10
- DOI:
- 10.3760/cma.j.issn.1006-9801.2007.10.002
- Key Word:
- 胃肿瘤;淋巴转移;小鼠,裸;模型,动物;移植肿瘤
Abstract： 目的 研究胃癌淋巴结转移动物模型的建立方法.方法 人类胃癌低分化细胞系SGC-7901体外培养、传代并扩增后,收集细胞行皮下种植成瘤,鼠间传代至第6代,以皮下肿瘤组织块原位种植于裸鼠胃壁建立动物模型.种植后第9周处死裸鼠,观察原位种植瘤生长、淋巴结转移及其他脏器转移情况,测定荷瘤裸鼠血清癌胚抗原(CEA)值.结果 原位移植瘤种植成功率100%,胃周淋巴结转移率93.3%,移植瘤可发生局部浸润及远处脏器转移,荷瘤裸鼠CEA值明显高于正常裸鼠(P＜0.01).结论 应用SGC-7901细胞系可成功建立胃癌的淋巴结转移动物模型.
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