You Position: Home > Paper
Construction and identification of RhoA specific siRNA expression vector in hepatocellular carcinoma cell
( views:388, downloads:12 )
- Author:
- No author available
- Journal Title:
- CANCER RESEARCH AND CLINIC
- Issue:
- 9
- DOI:
- 10.3760/cma.j.issn.1006-9801.2007.09.005
- Key Word:
- 肝肿瘤,实验性;遗传载体;RNA干扰
Abstract： 目的 构建靶向RhoA基因的RNA干扰(RNAi)载体.方法 根据人RhoA基因序列设计两条短双链,人工合成后,连接到真核表达载体Pgenesil-1中,构成Pgenesil-1-siRhoA1、Pgenesil-1-siRhoA2两个重组质粒,转入人肝癌细胞(HEPG2)中,Western blot方法检测RhoA-siRNA对RhoA表达的抑制效果,确定一个重组质粒进行下一步研究.结果 两质粒都有沉默RhoA基因的作用,Pgenesil-1-siRhoA1重组质粒作用更强烈一些,使用Pgenesil-1-siRhoA1质粒进行下一步研究.结论 Pgenesil-1-siRhoA重组质粒的成功构建,为研究RhoA与肝细胞癌侵袭转移的关系提供实验模型.
- [1]Erik, Sahai,Christopher J, Marshall.RHO-GTPases and cancer.[J].2002,2(2).
- [2]Yu JY,DeRuiter SL,Turner DL.RNA interference by expression of short-interfering RNAs and hairpin RNAs in mammalian cells.[J].2002,99(9).
- [3]Harborth J,Tuschl T,Elbashir SM,etc.Analysis of gene function in somatic mammalian cells using small interfering RNAs.[J].2002,26(2).
- [4]F, Michiels,J G, Collard.Rho-like GTPases: their role in cell adhesion and invasion.[J].1999,65.
- [5]G Y, Gillespie,L, Soroceanu,T J, Manning,etc.Glioma migration can be blocked by nontoxic inhibitors of myosin II.[J].1999,59.
- [6]Wossmann W,Wilda M,Borkhardt A,etc.Killing of leukemic cells with a BCR/ABL fusion gene by RNA interference (RNAi).[J].2002,21(37).
- [7]Kapadia SB,Brideau Andersen A,Chisari FV.Interference of hepatitis C virus RNA replication by short interfering RNAs.[J].2003,100(4).
- [8]Ridley AJ.Rho GTPases and cell migration.[J].2001,114(Pt.15).
- [9]O'Connor KL,Nguyen BK,Mercurio AM.RhoA function in lamellae formation and migration is regulated by the alpha6beta4 integrin and cAMP metabolism.[J].2000,148(2).