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Detection of micrometastasis in perioperative primary esophageal cancer patients using real-time quantitative reverse transcription-PCR
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- Author:
- No author available
- Journal Title:
- CANCER RESEARCH AND CLINIC
- Issue:
- 6
- DOI:
- 10.3760/cma.j.issn.1006-9801.2007.06.004
- Key Word:
- 食管肿瘤;肿瘤转移;癌胚抗原;围手术期
Abstract： 目的 探讨食管癌围手术期外周血微转移定量检测的理想途径,判断手术对微转移的影响程度.方法 对40例可手术的食管癌分别于手术前1天(记为B-1)、手术刚结束时(记为B0)和手术后第3天(记为B+3)取外周中心静脉血,分离单核细胞并提取总RNA,用实时荧光PCR技术定量检测癌胚抗原基因(CEA mRNA)表达水平,以胸部良性肿瘤患者和正常人为对照.结果 CEA mRNA拷贝值在B-1、B0和B+3取样时间点的中位值分别为1592、13 314、6221拷贝/ml.且在B0及B+3处的CEA mRNA表达水平都明显高于在B-1处的表达水平(P值分别为0.0001和0.0209).CEA mRNA表达水平在B0处高于B+3处,但差异无统计学意义(P=0.4396).结论 手术操作使得患者外周血的CEA mRNA表达水平明显增加,术后第3天有部分下降,但仍有部分患者存在高水平的CEA mRNA,表明手术促进微转移的发生,有必要行围手术期综合治疗.
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