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Study on apoptosis of human ovarian cancer cell induced by siRNA on Rab25
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- Author:
- No author available
- Journal Title:
- CANCER RESEARCH AND CLINIC
- Issue:
- 5
- DOI:
- 10.3760/cma.j.issn.1006-9801.2007.05.003
- Key Word:
- 卵巢肿瘤;基因,Rab25;RNA,小分子干扰;细胞增殖;细胞凋亡
Abstract： 目的 观察Rab25基因的小分子干扰RNA(siRNA)对肿瘤细胞的诱导凋亡作用.方法 成功构建Rab25基因的siRNA,稳定转染靶细胞,MTT法检测细胞增生率;流式细胞仪(FCM)检测细胞生长周期和细胞凋亡率;DNA琼脂糖凝胶电泳法(DNAladder)确定凋亡.结果 细胞生长明显受到抑制,增生能力下降;阻断细胞生长于G1期(转染Psuper/Rab25 siRNA组细胞G1期达到88.6%);细胞凋亡增加,转染Psuper/Rab25 siRNA组细胞凋亡率为12.3%,与A2780组细胞及转染空载体组细胞相比,差异有统计学意义(P＜0.01);DNA凝胶电泳可观察到细胞凋亡特异的DNA梯形带.结论 Rab25基因的siRNA可抑制对肿瘤细胞的生长,诱导其凋亡,可为卵巢癌基因治疗开辟新途径.
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