You Position: Home > Paper
The cloning and expressing of novel differential expression gene BQ135232 ORF
( views:169, downloads:4 )
- Author:
- No author available
- Journal Title:
- CANCER RESEARCH AND CLINIC
- Issue:
- 4
- DOI:
- 10.3760/cma.j.issn.1006-9801.2007.04.002
- Key Word:
- 计算生物学;新基因;克隆,分子
Abstract： 目的 研究差异表达新基因BQ135232的结构功能以及真核表达.方法 应用生物信息学方法分析BQl35232 mRNA基因序列,初步了解其基本性状,构建表达载体,转染真核细胞(CHO/dhfr-).结果 BQ135232具备作为免疫原的基本条件,构建表达载体pcDNA3-BQ-ORF-GPI,成功转染CHO/dhfr-.结论 完成差异表达新基因BQ135232的真核表达,为后续的蛋白功能研究奠定基础.
- [1].Construction of cDNA Library from NPC Tissue and Screening of Antigenic Genes[J].2006,3(1).
- [2]Engelmann H,Djafarzadeh R,Notohamiprodjo M,etc.GPI-anchored TIMP-1 treatment renders renal cell carcinoma sensitive to FAS-meditated killing.[J].2006,25(10).
- [3]Jenny C. Ho,Siu Tim Cheung,Mohini Patil,etc.Increased expression of glycosyl-phosphatidylinositol anchor attachment protein 1 (GPAA1) is associated with gene amplification in hepatocellular carcinoma[J].2006,119(6).
- [4]Borg NA,Burrows SR,McCluskey J,etc.The immunogenicity of a viral cytotoxic T cell epitope is controlled by its MHC-bound conformation.[J].2005,202(9).
- [5]Hiroshi, Yonemura,Takayuki, Imamura,Kenji, Soejima,etc.Preparation of recombinant alpha-thrombin: high-level expression of recombinant human prethrombin-2 and its activation by recombinant ecarin.[J].2004,135(5).
- [6]Ridgway D.,Kopchick JJ.,Haldankar R..Stable production of a human growth hormone antagonist from CHO cellsadapted to serum-free suspension culture[J].1999,15(3).
- [7]Ramaswamy, Narayanan.Bioinformatics approaches to cancer gene discovery.[J].2007,360.
- [8]Neil H, Segal,Nathalie E, Blachere,Jose A, Guevara-Pati?o,etc.Identification of cancer-testis genes expressed by melanoma and soft tissue sarcoma using bioinformatics.[J].2005,5.