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PKB-dependent pathway in colorectal cancer cell growth induction by bFGF
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- Author:
- No author available
- Journal Title:
- CANCER RESEARCH AND CLINIC
- Issue:
- 3
- DOI:
- 10.3760/cma.j.issn.1006-9801.2007.03.003
- Key Word:
- 成纤维细胞生长因子2;蛋白激酶B;肠肿瘤
Abstract： 目的 研究碱性成纤维细胞生长因子(bFGF)通过磷脂酰肌醇3激酶(PI3K)/蛋白激酶B(PKB)通路调控大肠癌细胞生长的信号转导机制.方法 MTT法分析bFGF对大肠癌LoVo细胞增生程度的影响;(γ-32P)ATP掺入法检测LoVo细胞株中PKB的活性;RT-PCR法检测LoVo细胞株中细胞周期素A(cyclinA)的表达;Western Blot检测PKB和cyclinA的表达.结果 bFGF以不同时间作用于LoVo细胞时,通过(γ-32P)ATP掺入法检测发现可提高细胞PKB活性.PKB的抑制剂LY294002预处理后再施加bFGF,可显著降低PKB的活性(P＜0.05),bFGF刺激LoVo细胞cyclinA蛋白表达都高于其他各组,LY294002抑制组蛋白明显降低,而PKB在各组中的表达无明显变化.结论 bFGF刺激大肠癌LoVo细胞后可通过依赖PI3K/PKB途径调节cyclin A的转录活性促进其表达,进而促进细胞增生.
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