You Position: Home > Paper
cDNA microarray in the gene expression pattern in lymphatic metastasis of colon carcinomas
( views:168, downloads:12 )
- Author:
- No author available
- Journal Title:
- CANCER RESEARCH AND CLINIC
- Issue:
- 10
- DOI:
- 10.3760/cma.j.issn.1006-9801.2006.10.005
- Key Word:
- 结肠癌;淋巴结转移;基因表达谱
Abstract： 目的 利用基因芯片技术分析伴与不伴淋巴结转移结肠癌组织中的差异表达基因.方法 将含有16 084条人类全长基因的PCR产物点样于特殊处理后的玻片上制备基因芯片.用反转录的方法,分别将两种荧光Cy3-dUTP和Cy5-dUTP标记结肠癌组织和淋巴结转移组织mRNA,以制备cDNA探针,并与芯片杂交.以Agilent荧光扫描仪扫描芯片上两种荧光信号,获得的荧光信号图像用计算机分析.结果 在16 084条基因中,结肠癌原发灶与转移淋巴结组织间存在差异表达的基因共999条,有537条基因出现显著性表达上调,462条基因出现显著性表达下调.在4例患者中出现共同表达上调基因44条,共同表达下调基因30条.包括癌基因、抑癌基因、黏附分子、基质金属蛋白酶、信号转导因子、细胞代谢和免疫相关基因等.结论 结肠癌转移是多基因作用的综合结果,转移相关基因表达谱的分析为预防和控制结肠癌的转移提供了新的思路与线索.
- [1]王朝夫,周晓燕,张太明,等.MLH1、MSH2基因 mRNA突变分析与遗传性非息肉性结直肠癌的基因诊断[J].2006,(1).
- [2]Kohonen Corish M,Delozier Blanchet C,Tops C,etc.Molecular characterization of the spectrum of genomic deletions in the mismatch repair genes MSH2, MLH1, MSH6, and PMS2 responsible for hereditary nonpolyposis colorectal cancer (HNPCC).[J].2005,44(2).
- [3]Leeman MF,Murray GI,Curran S.New insights into the roles of matrix metalloproteinases in colorectal cancer development and progression.[J].2003,201(4).
- [4]K W, Kinzler,M C, Nilbert,B, Vogelstein,etc.Identification of a gene located at chromosome 5q21 that is mutated in colorectal cancers.[J].1991,251(4999).
- [5]Nagle RB,Powell TJ,Bearss DJ,etc.Mutations in the G-quadruplex silencer element and their relationship to c-MYC overexpression, NM23 repression, and therapeutic rescue[J].2004,101(16).
- [6]Miyakawa I,Hamanaka R,Takai N,etc.Polo-like kinases (Plks) and cancer.[J].2005,24(2).
- [7]Sugiyama M,Atomi Y,Izumisato Y,etc.Matrix metalloproteinases may contribute compensationally to tumor invasion in T1 colorectal carcinomas.[J].2003,23(5B).
- [8]Solomon E.,Voss R.,Hall V.,etc.Chromosome 5 allele loss in human colorectal carcinomas[J].1987.
- [9]Aung PP,Nakayama H,Oue N,etc.Molecular-pathological prognostic factors of gastric cancer: a review.[J].2005,8(2).
- [10]Tachibana M,Fujimori T,Horiuchi H,etc.Dysfunction of p53 pathway in human colorectal cancer: Analysis of p53 gene mutation and the expression of the p53-associated factors p14ARF, p33ING1, p21WAF1 and MDM2.[J].2004,25(4).
- [11]Lubet, Ronald,Yian Wang,Zhongqiu Zhang,etc.Mouse Models Incorporating Alterations in the Major Tumor Suppressor Genes P53 and Pi6: Their Use in Screening for Potential Carcinogens, Developing Further Relevant Mouse Models, and Screening for Potential Chemopreventive and Chemotherapetutic Agents.[J].2005,31(1).