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The down expression genes in rat liver cancer induced by DEN

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Author:: No author available

Journal Title:: CANCER RESEARCH AND CLINIC

Issue:: 9

DOI:: 10.3760/cma.j.issn.1006-9801.2006.09.002

Key Word:: 二乙基亚硝胺;大鼠;肝癌组织;基因

Abstract：目的运用基因芯片技术,观察二乙基亚硝胺(DEN)诱导大鼠肝癌(含肝)组织与正常对照大鼠肝组织比较表达下调的基因.方法 DEN诱发大鼠肝癌,应用Affymetrix Rat 230A GeneChip进行检测.结果在芯片的15 710个基因中,剔除正常肝组织表达值读数在50以下的基因,正常肝脏基因表达大于肝癌基因的有248个,其中120个为EST片段,已知基因为128个.结论 DEN诱发大鼠肝癌的后基因组变化是十分复杂的,如何逐渐找到那些起着关键作用的基因,进一步阐释其在肝癌的发生、发展和转归中的作用,是今后工作的重点.

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