You Position: Home > Paper
Expression of inhibitors of apoptosis (IAPs) and its clinical significance in patients with non-small cell lung cancer
( views:298, downloads:4 )
- Author:
- No author available
- Journal Title:
- CANCER RESEARCH AND CLINIC
- Issue:
- 6
- DOI:
- 10.3760/cma.j.issn.1006-9801.2006.06.004
- Key Word:
- 凋亡抑制基因;非小细胞肺癌
Abstract： 目的探讨凋亡抑制基因(IAPs)家族在非小细胞肺癌(NSCLC)患者组织中的表达及其意义.方法采用半定量反转录聚合酶链反应(RT-PCR)及Western blot分别从mRNA和蛋白水平对36例NSCLC、36例肺部良性病变进行研究,检测生存蛋白(survivin),human IAP-1(hIAP-1),humanIAP-2(hIAP-2)及X chromosome-linked UAP(XIAP)表达情况.结果36例NSCLC组织中有32例survivin mRNA表达阳性,而对照组无一例阳性表达;26例癌组织中XIAP mRNA处于高表达,与对照组比较差异有统计学意义(P＜0.05);hIAP-1 mRNA仅仅在腺癌组织中高表达(P＜0.05),具有组织学类型特异性.Western blot显示在survivin mRNA阳性组织中均可检测到survivin蛋白的表达;XIAP和hIAP-1 mRNA高表达的组织同样可检测到相应蛋白.相关性分析表明只有hIAP-1的表达与NSCLC组织学类型相关.结论survivin mRNA高表达可以作为NSCLC辅助诊断的重要指标,hIAP-1可用来协助鉴定NSCLC亚型,survivin和XIAP在肿瘤基因治疗中具有潜在的应用价值和前景.
- [1]都昌胡,徐军.凋亡蛋白抑制剂家族研究进展[J].2005,(13).
- [2]R A, Olie,A P, Sim?es-Wüst,B, Baumann,etc.A novel antisense oligonucleotide targeting survivin expression induces apoptosis and sensitizes lung cancer cells to chemotherapy.[J].2000,60(11).
- [3]Welsh K,Stennicke HR,Salvesen GS,etc.Cleavage of human inhibitor of apoptosis protein XIAP results in fragments with distinct specificities for caspases.[J].1999,18(19).
- [4]Altieri DC.Survivin and apoptosis control.[J].2003,88(0).
- [5]C Y, Wang,M W, Mayo,R G, Korneluk,etc.NF-kappaB antiapoptosis: induction of TRAF1 and TRAF2 and c-IAP1 and c-IAP2 to suppress caspase-8 activation.[J].1998,281(5383).
- [6]Khaled W,Wheatley SP,Dodson H,etc.Aurora-B phosphorylation in vitro identifies a residue of survivin that is essential for its localization and binding to inner centromere protein (INCENP) in vivo.[J].2004,279(7).
- [7]Q L, Deveraux,J C, Reed.IAP family proteins--suppressors of apoptosis.[J].1999,13(3).