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Detection on aberrant CDKN2/p16 gene with serum DNA in colorectal cancer patients
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- Author:
- No author available
- Journal Title:
- CANCER RESEARCH AND CLINIC
- Issue:
- 4
- DOI:
- 10.3760/cma.j.issn.1006-9801.2006.04.005
- Key Word:
- 生物标志物;结直肠癌
Abstract： 目的寻找一种切实可行的检测肿瘤的生物标志物及其方法.方法对100例结直肠癌患者取血清,另取献血员19例,非肿瘤患者13例为对照;并另取癌组织13份,癌旁组织11份作对比观察,同时用三种方法进行检测.一为对p16基因甲基化分析;二为对其进行缺失分析,三为对其进行点突变的分析.结果患者血清异常甲基化为58%;缺失为14%;点突变为25%;癌组织之结果与血清相似;而癌旁组织显然较低.SSCP阳性者经序列分析证实有3例为错义突变,1例为移位,1例为同义突变.其中4例造成p16蛋白的缺失.作为肿瘤的生物标志物,三项联合应用其灵敏度为75%,特异性为96.87%,准确率为80.30%.比文献用CEA+CA19-9+CA72-4+CA242四项指标联合之灵敏度、特异性、准确率皆高,尤其是特异性.如果仅用甲基化加突变亦与之相当.结论血清DNA含量不高但能反映人体对肿瘤的负荷,实验对DNA的质和量的要求不是太高,能检出10-3的DNA片段,可用于临床诊断及大面积普查,并可用于对出院患者的长期随访.
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