The pathologic and immunologic changes of orthotopic rat bladder tumor were induced by HSP70-peptides complexes derived from autologous tumor
- Author:
- No author available
- Journal Title:
- CANCER RESEARCH AND CLINIC
- Issue:
- 2
- DOI:
- 10.3760/cma.j.issn.1006-9801.2006.02.004
- Key Word:
- 热疗;膀胱肿瘤;热休克蛋白70-肽复合物
Abstract： 目的研究用来自亚高温盆腔区域热疗后原位大鼠膀胱肿瘤的热休克蛋白70-肽复合物(HSP70-PC)对原位大鼠膀胱肿瘤的治疗作用机制.方法经尿道膀胱灌注N-甲基亚硝脲(MNU)制备出大鼠膀胱肿瘤动物模型.将动物随机分为A(肿瘤对照)组、B(HSP70-PC治疗)组、C(肿瘤细胞裂解液治疗)组,每组5只.皮下注射治疗1个月后检测膀胱及肿瘤湿重、肿瘤分期分级,TUNEL法检测肿瘤细胞凋亡指数(AI),免疫组化检测肿瘤细胞增生细胞核抗原指数(PCNA LI)、肿瘤组织中S-100蛋白阳性细胞率及CD8阳性细胞率、膀胱引流区域髂动脉及腹主动脉旁淋巴结中S-100蛋白阳性细胞率、脾脏S-100蛋白阳性细胞率及CD8阳性细胞率,ELISA法检测血清RatIFN-γ含量.结果膀胱及肿瘤湿重B组低于A,C组(P＜0.01);A,C组间差异无统计学意义(P=0.610).B组肿瘤分期低于A,C组(P＜0.01);A,C组间差异无统计学意义(P=1.00);肿瘤分级各组间差异均无统计学意义(P=1.00).B组肿瘤细胞AI、膀胱肿瘤组织中CD8阳性细胞率、血清RatIFN-γ含量均高于A,C组(P＜0.01);A,C组间差异均无统计学意义(P=0.870,0.597,0.979).脾脏S-100蛋白阳性细胞率及CD8阳性细胞率由高到低依次为B,C,A组(P＜0.01).肿瘤细胞PCNA LI、膀胱肿瘤组织中S-100蛋白阳性细胞率、淋巴结中S-100蛋白阳性细胞率三组之间差异均无统计学意义(P=0.808,0.718,0.847).结论皮下注射HSP70-PC可以上调原位的膀胱肿瘤大鼠的细胞免疫状态,促进肿瘤凋亡,但对肿瘤细胞增生无影响.
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