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EFFECT OF OCTREOTIDE ON GROWTH OF HUMAN GASTRIC CARCINOMA CELL LINE
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- Author:
- No author available
- Journal Title:
- CANCER RESEARCH AND CLINIC
- Issue:
- 6
- DOI:
- 10.3760/cma.j.issn.1006-9801.2004.06.002
- Key Word:
- 胃肿瘤;奥曲肽;增生;细胞凋亡
Abstract： 目的:研究生长抑素类似物奥曲肽(OCT)对胃癌SGC7901细胞的抑制作用及其作用机制.方法:不同浓度的OCT作用于体外培养的人胃癌SGC7901细胞,应用MTT法分析细胞生长抑制作用;流式细胞仪测定细胞周期分布和凋亡率;光镜观察细胞形态等.结果:OCT在0.005μg/mL～20.0μg/mL浓度范围内呈剂量依赖方式抑制胃癌SGC7901细胞的生长增生,OCT(0.25μg/mL～5.0μg/mL)作用48 h后,光镜可见SGC7901细胞呈典型的凋亡形态学改变;流式细胞仪检测出现凋亡峰,其测得的细胞凋亡率以及图像分析系统计算的细胞凋亡率与对照组相比均有显著性差异(P＜0.05).结论:OCT对人胃癌细胞增生具有抑制作用,其机制与诱导肿瘤细胞的凋亡有关.
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