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The Construction of an Eukaryotic Expression Vector pBBS212-AFP/HSP70
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- Author:
- No author available
- Journal Title:
- CANCER RESEARCH AND CLINIC
- Issue:
- 6
- DOI:
- 10.3760/cma.j.issn.1006-9801.2002.06.001
- Key Word:
- 重组基因;真核载体;肝癌
Abstract： 目的：构建真核表达载体pBBS212-AFP／HSP70，进行肝癌基因工程疫苗主动免疫的初步研究。方法：RT-PCR扩增AFP目的基因片段，与同酶切的pBBS212栽体连接，构建真核表达栽体pBBS212-AFP／HSP70。用碱裂解法和酶切对筛选阳性克隆进行鉴定。结果：成功构建pBBS212-AFP／HSP70真核载体，酶切显示AFP基因片段正确插入载体。结论：pBBS212-AFP／HSP70真核载体的构建为今后进行基因疫苗的实验研究奠定了良好的基础。
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