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The Apoptotic Susceptibility of Esophageal Cells to Arsenic Trioxide is Determined by Cellular Reactive Oxygen Species Level
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- Author:
- No author available
- Journal Title:
- CANCER RESEARCH AND CLINIC
- Issue:
- 5
- DOI:
- 10.3760/cma.j.issn.1006-9801.2002.05.003
- Key Word:
- 三氧化二砷;细胞凋亡;活性氧
Abstract： 目的：探索食管癌细胞株EC／CUHK1、EC／CUHK1(As-)对三氧化二砷(As2O3)促凋亡作用敏感性与细胞活性氧(reactive oxygen species，ROS)水平的关系。方法：以二甲蔡醌(DMNQ)孵育EC／CUHK1、EC／CUHK1(As-)细胞，双氢-乙酰乙酸二氯荧光黄(2，7-dichlorodmy-drofluorescein diacetate，DCFH-DA)及双氢罗丹明123(dihydrorhodamine123，DHR)捕获ROS，流式细胞仪检测两种细胞ROS水平的差异。As2O3单独或联用DMNQ孵育EC／CUHK1、EC／CUHK1(As-)细胞，流式细胞仪Tunel法分析两种细胞凋亡敏感性的差异及其在用药前后的变化。结果：EC／CUHK1的ROS水平明显高于EC／CUHK1(As-)，DMNQ可提高EC／CUHK1、EC／CUHK1(As-)的ROS水平，诱发EC／CUHK1(As-)对As2O3的敏感性，增强As2O3促EC／CUHK1(As-)细胞凋亡的效应。过氧化氢酶(catalase)可逆转DMNQ的效应。结论：食管癌细胞株EC／CUHK1、EC／CUHK1(As-)对As2O3促凋亡的敏感性决定于细胞固有的ROS水平。
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