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APOPTOSIS INDUCED BY TNF-α COMBINED WITH 5-Fu AND WITH EXPRESSION OF BCL-2 GENE IN COLON CANCER CELLS
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- Author:
- No author available
- Journal Title:
- CANCER RESEARCH AND CLINIC
- Issue:
- 6
- DOI:
- 10.3760/cma.j.issn.1006-9801.2001.06.006
- Key Word:
- 肿瘤坏死因子-α;5-氟尿嘧啶;凋亡;结直肠癌;bcl-2基因
Abstract： 目的:研究重组人肿瘤坏死因子α(rhTNF-α)和5-Fu联合诱导结肠癌细胞凋亡及其对bcl-2基因表达的影响.方法:应用MTT法检测TNF-α、5-Fu对结肠癌细胞SW-480的细胞毒作用;应用末端标记法(TUNEL法)研究结肠癌SW-480细胞凋亡;应用免疫组化法研究bcl-2基因的表达.结果:3×106U/L的rhTNF-α联合不同剂量的5-Fu作用于SW-480细胞48 h后对SW-480细胞生长具有协同抑制作用;联合应用3×106U/L的TNF-α,125 mg/L的5-Fu作用于SW-480细胞48 h后,TUNEL法显示染色阳性细胞比对照组显著增加(P＜0.01);免疫组化结果显示,联合用药组bcl-2基因表达阳性的细胞百分率较对照组显著减低(P＜0.01).结论:联合应用TNF-α、5-Fu有协同诱导SW-480细胞凋亡及抑制bcl-2基因的表达的作用.
- [1]M C, Raff.Social controls on cell survival and cell death.[J].1992,356(6368).
- [2]D C, Huang,S, Cory,A, Strasser.Bcl-2, Bcl-XL and adenovirus protein E1B19kD are functionally equivalent in their ability to inhibit cell death.[J].1997,14(4).
- [3]Micheau, Olivier,Solary, Eric.Sensitization of cancer cells treated with cytotoxic drugs to Fas-mediated cytotoxicity.[J].1997,89(11).
- [4]Schorkhuber M,Marian B,Partik G,etc.Inhibition of epidermal-growth-factor-receptor-dependent signalling by tyrphostins A25 and AG1478 blocks growth and induces apoptosis in colorectal tumor cells in vitro.[J].1999,125(7).
- [5]Peter Möller,Karin Koretz,Frank Leithäuser,etc.Expression of APO-1 (CD95), a member of the NGF/TNF receptor superfamily, in normal and neoplastic colon epithelium[J].1994,57(3).
- [6]R, Sharma,E, Adam,U, Schumacher.The action of 5-fluorouracil on human HT29 colon cancer cells grown in SCID mice: mitosis, apoptosis and cell differentiation.[J].1997,76(8).