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CIRCULATING CELL MICROMETASTASES OF OVARY CANCER
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- Author:
- No author available
- Journal Title:
- CANCER RESEARCH AND CLINIC
- Issue:
- 5
- DOI:
- 10.3760/cma.j.issn.1006-9801.2001.05.007
- Key Word:
- 角化蛋白19;卵巢癌;微转移;聚合酶链反应
Abstract： 目的:了解卵巢癌患者除直接侵袭或植入体腔外,是否还通过血液循环的渠道而转移.方法:以角蛋白19(K-19)-mRNA及癌胚抗原(CEA)-mRNA两基因的表达为标记物;应用巢式-反转录聚合酶链反应(Nested-RT-PCR)进行扩增,经PAGE后进行观察.结果:51例卵巢癌的骨髓标本中21例(41.18%)K-19为阳性,CEA阳性4例(7.84%),双阳性5例(9.8%);K-19加双项阳性为26例(50.98%),阴性21例(41.2%).22例周围血中K-19阳性3例(13.63%)、CEA 1例(4.55%),无双阳性,阴性1 8例(81.82%).经X2分析患者骨髓标本结果与正常对照间有显著性差异(P＜0.005),而患者周围血与对照组间则无明显差异(P＞0.05).临床分期之早晚及年龄之增长,其阳性率有上升趋势.病理型态之恶性程度高者微转移率较恶性程度低者为高.结论:以Nested-RT-PCR对K-19-mRNA表达的检测来判断卵巢癌的微转移是一种很灵敏的方法,凡阳性者必须及早采用较长时间化疗,并定期复查以观疗效以防复发.
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