You Position: Home > Paper
GROWTH REGULATING OF HUMAN PANCREAS CARCINOMA CELL JF305 BY SPHINGOSINE AND THE METABOLIZATION OF CALCIUM
( views:317, downloads:12 )
- Author:
- No author available
- Journal Title:
- CANCER RESEARCH AND CLINIC
- Issue:
- 3
- DOI:
- 10.3760/cma.j.issn.1006-9801.2001.03.003
- Key Word:
- 鞘氨醇;[Ca2+]i;人胰腺癌细胞株JF305
Abstract： 目的:观察鞘氨醇对人胰腺癌细胞株JF305的增殖作用;观察鞘氨醇对人胰腺细胞株JF305内Ca2+浓度的影响,探讨鞘氨醇的细胞信使作用.方法:细胞培养后,分别加入不同浓度的鞘氨醇,应用四甲基偶氮唑盐MTT比色法来观察细胞增殖程度;应用Fura-2/AM荧光比色测定[Ca2+]i结果:鞘氨醇促进人胰腺癌细胞株JF305的生长,促进JF305内Ca2+的生成,3 min内达峰值,并在0.5μmol/L～2.0 μmol/L时呈剂量依赖性.结论:鞘氨醇能促进人胰腺癌细胞株JF305增殖;鞘氨醇的信使作用是通过刺激Ca2+转运的和Ca2+的释放.
- [1]H, Zhang,N E, Buckley,K, Gibson,etc.Sphingosine stimulates cellular proliferation via a protein kinase C-independent pathway.[J].1990,265.
- [2]H, Zhang,N N, Desai,J M, Murphey,etc.Increases in phosphatidic acid levels accompany sphingosine-stimulated proliferation of quiescent Swiss 3T3 cells.[J].1990,265.
- [3]Sato K.,Watanabe T.,Tomura H.,etc.Sphingosine 1-phosphate stimulates proliferation and migration of human endothelial cells possibly through the lipid receptors, Edg-1 and Edg-3[J].2000,348(Pt.1).
- [4]Carpio LC,Stephan E,Kamer A,etc.Sphingolipids stimulate cell growth via MAP kinase activation in osteoblastic cells.[J].1999,61(5).
- [5]A V, Alessenko.The role of sphingomyelin cycle metabolites in transduction of signals of cell proliferation, differentiation and death.[J].2000,13(2).
- [6]Ghosh, T.K.,Bian, J..Intracellular calcium release mediated by sphingosine derivatives generated in cells.[J].1990,248(4963).
- [7]C P, Chao,S J, Laulederkind,L R, Ballou.Sphingosine-mediated phosphatidylinositol metabolism and calcium mobilization.[J].1994,269(8).