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THE CLINICAL SIGNIFICANCE ON DETECTION OF CYTOKERATIN 19 mRNA IN PERIPHERAL VENOUS BLOOD FROM PATIENTS WITH LUNG CANCER
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- Author:
- No author available
- Journal Title:
- CANCER RESEARCH AND CLINIC
- Issue:
- 2
- DOI:
- 10.3760/cma.j.issn.1006-9801.2001.02.005
- Key Word:
- 肺癌;反转录聚合酶链反应;细胞角蛋白19;转移
Abstract： 目的:建立一种敏感的方法来检测肺癌患者外周血循环癌细胞,并探讨其临床应用的意义.方法:采用反转录聚合酶链反应(RT-PCR)技术检测肺癌患者外周血CK19-mRNA的表达情况.结果:40例肺癌外周血标本中16例(40%)CK19-mRNA阳性.15例肺良性病变中1例(6.6%)阳性,5例正常人为阴性.10例有转移的肺癌患者,其外周血CK19-mRNA均为阳性(100%),30例检测时尚无转移证据者,有6例阳性(20%),经过半定量分析,两组CK19-mRNA在量上差别有显著性.结论:CK19-mRNA可作为一个标志物来检测肺癌患者外周血微转移;定量检测外周血CK19-mRNA有助于诊断肺癌的血行转移;定期检测外周血CK19-mRNA,尚可作为疗效的观察指标.
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