Abstract： [Objective] To observe the characteristics of spectral-domain optical coherence tomography (SD-OCT) and fundus autofluorescence (FAF) in acute and chronic central serous chorioretinopathy (CSC).[Methods] Seven-three eyes of 67 patients with CSC diagnosed by slit-lamp microscopy,fundus photochromy,fundus fluorescein angiography (FFA) and indocyanine green angiography were enrolled in this study.All the patients were examined for FAF and SD-OCT.The patients were divided into acute CSC group (37 patients,37 eyes) and chronic CSC group (30 patients,36 eyes) according to the clinical features and FFA images.According to the OCT feature in retinal detachment area,they were divided into three categories,which including intact,non-intact and atrophy outer segment,respectively.According to the FAF characteristics,they were divided into hyper-FAF,hypo-FAF and mixed type,respectively.The characteristics of SD-OCT and FAF of both acute and chronic CSC patients were evaluated and analyzed.[Results] In acute CSC group,19 eyes (51.35％) were hypo-FAF,18 eyes (48.65％) were hyper-FAF.In chronic CSC group,two eyes (5.56％) were hypo-FAF,16 eyes (44.44％) were hyper-FAF,and 18 eyes (50.00％) were mixed type.There was significant difference between both groups (x2=31.872,P=0.000).The SD-OCT results showed that in acute group,15 eyes (40.54％) were intact outer segment,18eyes (48.65％) were non-intact outer segment,and four eyes (10.81％) were atrophy outer segment.In chronic group,five eyes (13.89％) were intact outer segment,17 eyes (47.22％) were non-intact outer segment,and 14 eyes (38.89 ％) were atrophy outer segment.There was significant difference between both groups (x2 =10.572,P=0.005).[Conclusion]s The FAF characteristics of acute and chronic CSC mainly manifests hypo-FAF and mixed type,respectively.The OCT characteristics of acute CSC mainly manifests intact outer segment and non-intact outer segment,but non-intact outer segment and atrophy outer segment in chronic CSC.