Abstract： Objective To observe the safety and efficacy of posterior vitreous detachment (PVD) induced by combined intravetreal injection of lysine-plasminogen and reteplase in rabbits. Methods Fifteen healthy New Zealand rabbits were divided into three groups with five rabbits in each. Take the right eyes as experimental eyes, while the left eyes as the control. The experimental eyes of three groups received combined intravetreal injection of 1250 μg/ml lysine-plasminogen at 0. 1 ml dose and 104U,3×104 U, 105U reteplase at 0.05 ml dose recpectively, while the control eyes were injected intravetreally with 0. 15 ml balanced salt solution. The conjunctiva, anterior chamber, lens, vitreous body, and retina were examined by slit lamp microscope and + 120D preset lens. The retinal function was examined by electroretinogram (ERG). Results All the experimental eyes had PVD. The results of optical microscope showed that no change in retinal structure was found in the control group and 104 U reteplase group, clear retinal hierarchical but decreased ganglion cells and kernel layer cells were found in 3×104 U reteplase group, only retinal pigment epithelium layer but no normal retinal structure was observed in 105 U reteplase group. The results of ERG showed that compared the maximum mixed reaction of a and b wave amplitude in control group and reteplase group respectively, the difference was not statistically siginificant between 104U reteplase group and control group(a wave:t = 0. 881, -1. 773,0. 809sb wave:t =- 0. 223,-0. 441,1. 400;P>0. 05), the differences were statistically siginificant between 3 × 104 U (a wave:t= -3. 20,b wave:t =- 4.182,-4.103), 105 U reteplase group(a wave:t=-0. 737,b wave:t=- 15. 150,6. 597) and control group(P<0. 05). The control eyes didn't had PVD. Conclusion Combined intravetreal injection of lysineplasminogen and reteplase can induce complete PVD, and no damage to the retinal structure in rabbits.