The dynamic expression of nestin and glial fibrilary acidic protein in the development of retina in rats

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Author:
XUE Li-ping(Deparment of Ophthalmology, Yunnan NO. 2Provincial People's Hospital, Kunming 650031 , China)
DING Peng()
JIANG Chun-guang(Deparment of Ophthalmology, Yunnan NO. 2Provincial People's Hospital, Kunming 650031 , China)
()
()
()
Journal Title:
CHINESE JOURNAL OF OCULAR FUNDUS DISEASES
Issue:
Volume 26, Issue 03, 2010
DOI:
10.3760/cma.j.issn.1005-1015.2010.03.17
Key Word:
Intermediate filament proteins/physiology;Glial Fibrillary Acidic Protein/physiology;Retina/growth & development;Animal experimentation

Abstract: Objective To observe the dynamic expression of nestin and glial fibrilary acidic protein(GFAP) in the development of retina in rats. Methods In 48 Wistar rats, 24 were divided into 8 groups with 3 rats in each according to their age (1 day, 1 week, and 2, 3, 4, 7, 12, and 20 weeks old). The sagittal freezing sections of the eye were made; nestin/glutamine synthetase (GS) and GFAP/GS were stained by immunofluorescence and were observed under the confocal microscope. Total RNA was extracted from 18 rats which were divided into 6 groups according to the age (1 day, 1 week, and 2, 3, 4, and 12 weeks old) with 3 rats in each. The expression of nestin, GAFA and GS mRNA were detected by real-time postnatal day 7-12 rats; the expression of nestin and GFAP was detected by immunostaining study. Double immunofluorescence was carried out between nestin/GS and GFAP/GS. Results One day after the birth,nestin positive ceils were found in the whole retinal neuroblast layers with elongated retinal progenitor cells;the GFAP positive astrocytes were observed in the inner retina. One week after the birth, Mailer glial cells expressed GS and nestin but not GFAP; GFAP positive cells localized in the inner retina. Two to 12 weeks GFAP in astrocytes didn't change much. The Mailer cells expressed nestin but no GFAP in vitro. The expression of nestin and GFAP mRNA in retina was accordant with the results of immunofluorescence ceils in neonatal and adult rats.

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