The dynamic expression of nestin and glial fibrilary acidic protein in the development of retina in rats

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XUE Li-ping(Deparment of Ophthalmology, Yunnan NO. 2Provincial People's Hospital, Kunming 650031 , China)
DING Peng()
JIANG Chun-guang(Deparment of Ophthalmology, Yunnan NO. 2Provincial People's Hospital, Kunming 650031 , China)
Journal Title:
Volume 26, Issue 03, 2010
Key Word:
Intermediate filament proteins/physiology;Glial Fibrillary Acidic Protein/physiology;Retina/growth & development;Animal experimentation

Abstract: Objective To observe the dynamic expression of nestin and glial fibrilary acidic protein(GFAP) in the development of retina in rats. Methods In 48 Wistar rats, 24 were divided into 8 groups with 3 rats in each according to their age (1 day, 1 week, and 2, 3, 4, 7, 12, and 20 weeks old). The sagittal freezing sections of the eye were made; nestin/glutamine synthetase (GS) and GFAP/GS were stained by immunofluorescence and were observed under the confocal microscope. Total RNA was extracted from 18 rats which were divided into 6 groups according to the age (1 day, 1 week, and 2, 3, 4, and 12 weeks old) with 3 rats in each. The expression of nestin, GAFA and GS mRNA were detected by real-time postnatal day 7-12 rats; the expression of nestin and GFAP was detected by immunostaining study. Double immunofluorescence was carried out between nestin/GS and GFAP/GS. Results One day after the birth,nestin positive ceils were found in the whole retinal neuroblast layers with elongated retinal progenitor cells;the GFAP positive astrocytes were observed in the inner retina. One week after the birth, Mailer glial cells expressed GS and nestin but not GFAP; GFAP positive cells localized in the inner retina. Two to 12 weeks GFAP in astrocytes didn't change much. The Mailer cells expressed nestin but no GFAP in vitro. The expression of nestin and GFAP mRNA in retina was accordant with the results of immunofluorescence ceils in neonatal and adult rats.

  • [1]Newman EA.A purinergic dialogue between gila and neurons in the retina.Novartis Found Symp,2006,276:193-202.
  • [2]Lendahl U,Zimmerman LB,Mckay RDG.CNS stem cells express a new class of intermediate filament protein.Cell,1990,60:585-595.
  • [3]Benvenuti S,Cramer R,Quinn CC,et al.Differential proteome analysis of rcplicativc senescence in rat embryo fibrobl.Mol Cell Proteomics,2002,1:280-292.
  • [4]Craig CG,Tropepe V,Morshead CM,et al.In vivo growth factor expansion of endogenous subependymal neural precursor cell populations in the adult mouse brain.J Neurosci,1996,16:2649-2658.
  • [5]Mckay RD.Stem cell biology and neurodegenerative disease.Philos Trans R Soc Lond B Biol Sci,2004,359:851-856.
  • [6]Tropepe V,Colts BL,Chiasson BJ,et al.Retinal stem ceils in the adult mammalian eye.Science,2000,287:2032-2036.
  • [7]Walcott JC,Provis JM.Mailer cells express the neuronal progenitor cell marker nestin in both differentiated and undifferentiated human foetal retina.Clin Exp Ophthalmol,2003,31:246-249.
  • [8]Fischer AJ,Omar G.Transitin,a nestin-related intermediate filament,is expressed by neural progenitors and can be induced in Müller glia in the chicken retina.J Comp Neurol,2005,484:1-14.
  • [9]Monnin J,Morand-Villeneuve N,Michel G,et al.Production of neurospheres from mammalian Müller cells in culture.Neurosci Lett,2007,421:22-26.
  • [10]Yin X,Chen C,Yuan R,et al.An immunofluorescencehistochemistry study of the Nogo receptor in the rat retina during postnatal development.Ann Ophthalmol,2007,39:140-144.
  • [11]吴继红,易苗英,黄倩.四种中间丝蛋白在人鼠视网膜发育过程中表达的时间顺序和结构特征.中华眼科杂志,2004,40:765-769.
  • [12]Henderson RG,Fernald RD.Timing and location of rhodopsin expression in newly born rod photoreceptors in the adult tcleost retina.Brain Res Dcv Brain Res,2004,151:193-197.
  • [13]Canto-Soler MV,Huang H,Romero MS,et al.Transcription factors CTCF and Pax6 are segregated to different cell types during retinal cell differentiation.Dev Dyn,2008,237:758-767.
  • [14]Marquardt T,Gruss P.Generating neuronal diversity in the retina:one for nearly all.Trends Neurosci,2002,25:32-38.
  • [15]Mehta V,Sernagor E.Receptive field structure-function correlates in developing turtle retinal ganglion cells.Eur J Neurosci,2006,24:787 794.
  • [16]金燕,李根林.张晓光.视网膜神经细胞发育发生的研究现状.国外医学眼科学分册,2002,26:365-369.
  • [17]Hegde GV,James J,Das AV,et al.Characterization of early retinal progenitor micro-environment:presence of activities selective for the differentiation of retinal ganglion cells and maintenance of progenitors.Exp Eye Res,2007,84:577-590.
  • [18]Derouiche A.Possible role of the Müller cell in uptake and metabolism of glutamate in the mammalian outer retina.Vision Res,1996,36:3875-3878.
  • [19]Mcssam CA,Hou J,Major EO.Coexprcssion of nestin in neural and glial cells in the developing human CNS defined by a humanspeeific anti-nestin antibody.Exp Neurol,2000,161:585-596.
  • [20]Ahmad I,Tang L,Pham H.Identification of neural progenitors in the adult mammalian eye.Biocbem Biophys Res Commun,2000,270:517-521.
  • [21]Tropepe V,Coles BI.,Chiasson BJ,et al.Retinal stem cells in the adult mammalian eye.Science,2000,287:2032-2036.
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