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Effect of batroxobin on the blood-retinal barrier and vascular endothelial growth factors in diabetes rats
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF OCULAR FUNDUS DISEASES
- Issue:
- 1
- DOI:
- 10.3760/j.issn:1005-1015.2006.01.005
- Key Word:
- 糖尿病视网膜病;糖尿病;血视网膜屏障;内皮,血管;内皮生长因子
Abstract： 目的研究巴曲酶对糖尿病大鼠血视网膜屏障以及视网膜血管内皮生长因子(VEGF)表达的影响.方法将60只大鼠用链尿佐菌素腹腔注射制成糖尿病大鼠模型,分成糖尿病组(n=20)、40 mg/kg巴曲酶注射组(n=20)和20 mg/kg巴曲酶注射组(n=20).另25只正常大鼠为正常对照组.7 d后处死全部大鼠,通过伊凡思蓝法观察各组大鼠血视网膜屏障情况,酶连免疫吸附法分析视网膜总蛋白中的VEGF含量,比较各组结果.结果正常对照组大鼠视网膜内渗漏的伊凡思蓝含量明显低于另外3个糖尿病大鼠组(P＜0.01),不同剂量巴曲酶治疗组之间伊凡思蓝含量无明显差异(P＞0.05),巴曲酶治疗的2组大鼠伊凡思蓝含量均比糖尿病组大鼠低(P＜0.05).正常对照组大鼠、不同剂量巴曲酶注射的2组大鼠视网膜内VEGF含量明显低于糖尿病大鼠(P＜0.01);正常对照组视网膜内VEGF含量与20 mg/kg巴曲酶注射组比较无明显差异(P=0.06);40 mg/kg巴曲酶注射组视网膜内VEGF含量比正常对照组低(P=0.01);不同剂量的巴曲酶治疗组之间VEGF含量无明显差异(P=0.78).结论巴曲酶治疗减轻了糖尿病大鼠血视网膜屏障功能的损伤,降低了VEGF的表达,提示巴曲酶对糖尿病大鼠血视网膜屏障功能有一定的保护作用.
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