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Effect of thiazolidinediones on early retinopathy in rats with experimental diabetes
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF OCULAR FUNDUS DISEASES
- Issue:
- 1
- DOI:
- 10.3760/j.issn:1005-1015.2006.01.003
- Key Word:
- 糖尿病视网膜病;内皮,血管;内皮生长因子;链脲佐菌素;疾病模型,动物
Abstract： 目的观察噻唑烷二酮类(TZDs)药物罗格列酮和吡格列酮对实验性糖尿病大鼠早期视网膜病变的影响.方法在40只大鼠中利用链脲佐菌素(STZ)一次性腹腔注射制作糖尿病大鼠模型36只,另4只为正常对照组.将生存至病程6个月以上的24只大鼠分组,分别行玻璃体腔注射罗格列酮和吡格列酮(每组各10只),另4只不注射药物作为糖尿病阳性对照.眼球石蜡切片进行血管内皮生长因子(VEGF)免疫组织化学链霉卵白素生物素复合物(SABC)法、VEGF mRNA原位杂交、末端脱氧核糖核酸转移酶介导的dUTP缺口末端标记(TUNEL)法检测细胞凋亡,并比较注射TZDs大鼠和对照组大鼠视网膜VEGF表达及细胞凋亡的不同.结果正常大鼠视网膜VEGF免疫组织化学染色及原位杂交检测结果均为阴性.注射罗格列酮和吡格列酮大鼠与糖尿病阳性对照大鼠相比,视网膜VEGF表达阳性细胞率有所下降,但VEGF mRNA的阳性表达以及视网膜细胞凋亡检测结果无明显差别.结论TZDs药物罗格列酮和吡格列酮对STZ诱导的糖尿病大鼠视网膜VEGF的总体表达具有一定的抑制作用,但并不影响该细胞因子在视网膜上的生成.
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