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Histopathological study after radial optic neurotomy in pigs' eyes in vivo
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF OCULAR FUNDUS DISEASES
- Issue:
- 1
- DOI:
- 10.3760/j.issn:1005-1015.2005.01.005
- Key Word:
- 视神经/外科学;视神经/病理生理学;猪
Abstract： 目的观察活体猪眼行放射状视神经切开术(RON)后的病理改变过程,为临床RON安全性提供实验依据.方法健康小型猪共12只,其中8只双眼行RON后,分别于1、3、7、48 d处死,每天各处死2只猪;2只猪一只眼行RON,另一只眼作正常对照;2只猪一只眼行RON,另一只眼行单纯玻璃体切除,于120 d后处死.取出眼球后常规制作石蜡组织切片行苏木精-伊红染色、Masson三色染色或Luxol坚固蓝染色,光学显微镜下对不同水平的视神经断面进行观察.结果所有眼球大血管壁未被破坏,球后视神经的软脑膜保持完整.手术后第1天可见创口形成,局部出血向周围及后方浸润,神经纤维髓鞘脱失所致的空泡样改变局限在创口内.手术后第3天时可见空泡样改变范围扩大.手术后第7天创口成纤维细胞密集,神经胶质细胞增生及分散的色素颗粒存在,浸润的炎性细胞以淋巴细胞和单核细胞为主.手术后第48天视盘创口胶原成分填充,切开深度未及的后方视神经局部神经胶质细胞密集,坚固蓝染色浅淡,边界清晰,其范围部分越过中线.手术后第120天创伤下方部位呈现局部视神经萎缩性改变.正常眼及单纯玻璃体切除眼切片光学显微镜观察未见病理改变.结论正常活体猪眼行RON后最终形成局部视神经萎缩,一定程度上手术本身是安全的.
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