You Position: Home > Paper
Detection of multiple apoptosis related proteins in retinoblastoma
( views:309, downloads:16 )
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF OCULAR FUNDUS DISEASES
- Issue:
- 4
- DOI:
- 10.3760/j.issn:1005-1015.2004.04.005
- Key Word:
- 视网膜母细胞瘤/病因学;免疫组织化学;蛋白质p53;基因,bcl-2
Abstract： 目的探讨survivin在视网膜母细胞瘤(RB)中的表达及其与RB临床分期、组织分化程度及p53、bcl-2蛋白表达的关系. 方法应用抗survivin、p53和bcl-2单克隆抗体,采用链霉菌抗生物素蛋白-过氧化酶免疫组织化学法对38例RB常规石蜡标本行survivin、p53和bcl-2蛋白表达的测定,对6例正常视网膜组织行survivin表达的测定. 结果 38例RB组织中20例survivin蛋白呈阳性表达,占52.6%,6例正常视网膜组织内均无survivin蛋白表达,两组比较差异有显著性的意义(P＜0.05).survivin的阳性表达与患者的性别、临床分期、组织学分型无关(P＞0.05).38例RB组织中,25例p53阳性表达,18例bcl-2阳性表达,阳性表达率分别为65.8%和47.4%.p53、 bcl-2阳性表达组中survivin阳性表达率均显著高于p53、 bcl-2阴性表达组(P均<0.05). 结论 survivin在RB中表达上调,提示该基因可能在RB的发生发展中起一定作用;survivin、p53和bcl-2可能通过相互作用共同参与RB的发生发展.
- [1]任力蒙,张成.视网膜母细胞瘤细胞分化程度对扩散和存活预后的影响[J].2001,(1).
- [2]卢颖,王晋芬,李光.视网膜母细胞瘤患者的预后因素分析[J].2003,(3).
- [3]任若瑾,李彬.视网膜母细胞瘤分子生物学研究进展[J].2003,(2).
- [4]Murphy M,Hoffman WH,Zilfou JT,etc.Transcriptional repression of the anti-apoptotic survivin gene by wild type p53.[J].2002,277(5).
- [5]Kimura W,Jinfeng M,Takeshita A,etc.Histopathological study of intraductal papillary mucinous tumor of the pancreas: special reference to the roles of Survivin and p53 in tumorigenesis of IPMT.[J].2002,32(2).
- [6]Cotsonis G,Lawson D,Santoianni R,etc.Survivin expression in ovarian carcinoma: correlation with apoptotic markers and prognosis.[J].2003,16(6).
- [7]Mirza Asra,McGuirk Marnie,Hockenberry TishN,etc.Human survivin is negatively regulated by wild-type p53 and participates in p53-dependent apoptotic pathway.[J].2002,21(17).
- [8]Li F.Survivin study: what is the next wave?[J].2003,197(1).
- [9]Das A,Teo J,Tan WL,etc.Expression of survivin in primary glioblastomas.[J].2002,128(6).