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Matrix metalloproteinase in human diabetic and non-diabetic vitreous
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF OCULAR FUNDUS DISEASES
- Issue:
- 3
- DOI:
- 10.3760/j.issn:1005-1015.2001.03.009
- Key Word:
- 糖尿病视网膜病;视网膜脱离;基质金属蛋白酶/分析;视网膜
Abstract： 目的比较糖尿病视网膜病变(diabetic vitreoretinopathy, DR)及非糖尿病视网膜病变(non-DR)患者玻璃体中基质金属蛋白酶(matrix metalloproteinase, MMP)表达及活性;并探讨MMP表达的相关因素. 方法以31例DR、17例non-DR(9例黄斑裂孔,8例视网膜前膜)玻璃体为样本,采用明胶酶谱(Zymography)技术检测样本中MMP-2、9,并用Western印迹杂交方法证实MMP-2、9表达. 结果所有样本中都检测到MMP-2表达,其中DR玻璃体中MMP-2活性略高于non-DR,但差别无显著性意义.45.2%的DR玻璃体样本中有MMP-9表达,而non-DR玻璃体样本中无一例有 MMP-9表达.DR玻璃体样本中,部分性玻璃体后脱离(posterior vitreous detachment, PVD)者MMP-9表达的阳性率(66.7%)明显高于完全性PVD者(15.4%)(P＜0.01).Western印迹杂交结果进一步证实了MMP-2、9表达. 结论 DR及non-DR玻璃体样本中MMP-2活性无明显差异;non-DR玻璃体样本中未见MMP-9表达,而DR玻璃体样本中MMP-9表达率与部分性PVD密切相关.MMP-9可能与DR的发生有关.
- [1]D, Brown,H, Hamdi,S, Bahri,etc.Characterization of an endogenous metalloproteinase in human vitreous.[J].1994,13.
- [2]Dr. Marsha A. Moses.The Regulation of Neovascularization by Matrix Metalloproteinases and Their Inhibitors[J].1997,15(3).
- [3]Jalkh A.,Takahashi M.,Topilow H. W.,etc.Prognostic Value of Vitreous Findings in Diabetic Retinopathy[J].1982.
- [4]S. L. Parsons,S. A. Watson,P. D. Brown,etc.Matrix metalloproteinases[J].1997,84(2).
- [5]M E, Fini,M T, Girard,M, Matsubara,etc.Unique regulation of the matrix metalloproteinase, gelatinase B.[J].1995,36(3).
- [6]P, Hiscott,H A, Waller,I, Grierson,etc.The extracellular matrix of reparative tissue in the vitreous: fibronectin production in proliferative diabetic retinopathy membranes.[J].1993,7 ( Pt 2).
- [7]A M, Abu El-Asrar,L, Dralands,M, Veckeneer,etc.Gelatinase B in proliferative vitreoretinal disorders.[J].1998,125(6).