The effects of HSP70/CD80 DNA vaccine on airway inflammation and hyperresponsiveness of asthmatic mice

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Author:
CHENG Zheng-yan(Department of Pathology, Luzhou Medical College, Luzhou 646000, China)
SHI Xiao-ling(Laboratory of Infection and Immunology, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, China)
LI Yan(Department of Pathology, Luzhou Medical College, Luzhou 646000, China)
LI Guo-ping()
LI Shi-ning(Department of Pathology, Luzhou Medical College, Luzhou 646000, China)
ZHONG Sen()
CHEN Zhuang(Department of Pathology, Luzhou Medical College, Luzhou 646000, China;Laboratory of Infection and Immunology, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, China)
Journal Title:
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
Issue:
Volume 29, Issue 11, 2009
DOI:
10.3760/cma.j.issn.0254-5101.2009.11.005
Key Word:
HSP70; CD80; Asthma; Airway inflammation; Airway hyperresponsiveness

Abstract: Objective To investigate the effect of HSP70/CD80 DNA vaccine on the airway inflammation and hyperresponsiveness of asthmatic mice. Methods Forty female healthy BALB/c mice were randomly divided into 4 groups; saline group, asthma group, pcDNA3. 1 plasmid control group, and prevention group with HSP70/CD80 DNA vaccine, with 10 mice in each group. The mice were immunized by intramuscular( i. m. ) injection with HSP70/CD80 DNA vaccine before sensitization and challenge with ovalbumin. Then, the murine model of allergic asthma was made with injection of ovalbumin intraperitoneal ( i. p. ) , and inhalation of ovalbumin. Before mice were sanctified, their airway hyperresponsiveness( AHR) was measured. After mice were sanctified, bronchoalveolar lavage fluid( BALF) was obtained and cytokine IL-13 and IFN-γ were measured. And the lung histology and histochemistry were examined. Results Compared with mice in asthma and pcDNA3. 1 group, mice in vaccine group showed significantly reduced airway inflammation (P<0. 05) and AHR (P<0. 05). IFN-γ content in BALF were increased in mice from vaccine group compared with the asthma group and the pcDNA3. 1 group ( P <0. 05) , and IL-13 content in BALF were decreased. Conclusion HSF70/CD80 DNA vaccine can reduce airway inflammation and hyperresponsiveness in asthmatic mouse and this chimerical plasmid could be a candidate vaccine to prevent asthma.

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