Effects of butyrate on matrix metalloproteinase-3 expression in intestinal epithelial cell line No.6

( views:171, downloads:0 )
Author:
ZHU Jiang-hu(Department of Neonatology, Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical College, Wenzhou 325027, China)
ZHU Min-li(Department of Neonatology, Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical College, Wenzhou 325027, China)
YE Xiao-hua(Department of Neonatology, Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical College, Wenzhou 325027, China)
CHEN Chun(Department of Neonatology, Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical College, Wenzhou 325027, China)
LIN Jin()
LI N Zhen-lang(Department of Neonatology, Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical College, Wenzhou 325027, China)
Journal Title:
CHINESE JOURNAL OF PERINATAL MEDICINE
Issue:
Volume 14, Issue 08, 2011
DOI:
10.3760/cma.j.issn.1007-9408.2011.08.005
Key Word:
Enterocolitis, necrotizing;Cell line;Matrix metalloproteinase 3;Butyrates

Abstract: Objective To investigate the effects of butyrate on the expression of matrix metalloproteinase-3 (MMP-3) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in intestinal epithelial cell line No. 6 (IEC-6) cells. Methods IEC-6 cells of logarithmic growth phase were divided into three groups according to different concentrations of sodium butyrate being used. Low-concentration group, the final concentration of sodium butyrate in medium was 2 mmol/L; high-concentration group, the final concentration of sodium butyrate in medium was 14 mmol/L and control group with no sodium butyrate in medium. After cultured for 24 hours, expression of MMP-3 and TIMP-1 mRNA in IEC-6 cells was measured by reverse transcription-polymerase chain reaction. After cultured for 48 hours, Western blot was employed to examine the expression of MMP-3 and TIMP-1 protein in IEC-6 cells. The differences between groups were compared by ANOVA. Results Expression level of MMP-3 mRNA in group treated with butyrate in concentration of 14 mmol/L was 0. 566±0. 140,inactive form protein of MMP-3 in the group was 0. 756±0. 128, and expression level of active form protein was 0. 255±0. 057, which were higher than those of control group (0. 166±0. 041, 0. 312± 0. 057, 0. 042 ± 0. 038) (P<0.01, respectively). While, there were no significant differences of those indexes between the control group and low-concentration group. In the high and low-concentration and control group, the levels of TIMP-1 mRNA were 0. 427±0. 042, 0. 383±0. 043 and 0. 355±0. 048,the expression of TIMP-1 protein were 0. 576±0. 140, 0. 524±0. 123 and 0. 355±0. 062, there were no singnificant difference among the three groups (F= 1.97 and 3.10, P> 0.05). Conclusions High concentration sodium butyrate could induce the expression of MMP-3 in IEC-6 cells, while not at low concentration.

  • [1]Peng L,He Z,Chen W,et al.Effects of butyrate on intestinal barrier function in a Caco-2 cell monolayer model of intestinal barrier.Pediatr Res,2007,61:37-41.
  • [2]Lin J,Nafday SM,Chauvin SN,et al.Variable effects of short chain fatty acids and lactic acid in inducing intestinal mucosal injury in newborn rats.J Pediatr Gastroenterol Nutr,2002,35:545-550.
  • [3]朱将虎,林振浪,林锦,等.丁酸钠对大鼠小肠上皮细胞株-6细胞增殖和凋亡的影响.中华围产医学杂志,2008,11:115-118.
  • [4]PenderSL,Braegger C,Gunther U,et al.Matrix metalloproteinases in necrotising enterocolitis.Pediatr Res,2003,54:160-164.
  • [5]CockleJV,Gopichandran N,Walker JJ,et al.Matrix metalloproteinases and their tissue inhibitors in preterm perinatal complications.Reprod Sci,2007,14:629-645.
  • [6]Lin J.Too much short chain fatty acids cause neonatal necrotizing enterocolitis.Med Hypotheses,2004,62:291-293.
  • [7]Kleiner DE,Stetler-Stevenson WG.Matrix metalloproteinases and metastasis.Cancer Chemother Pharmacol,1999,43 Suppl:S42-S51.
  • [8]Tonti GA,Mannello F,Cacci E,et al.Neural stem cells at the crossroads:MMPs may tell the way.Int J Dev Biol,2009,53:1-17.
  • [9]Pender SL,MacDonald TT.Matrix metalloproteinases and the gut-new roles for old enzymes.Curr Opin Pharmacol,2004,4:546-450.
  • [10]Cruz-Munoz W,Khokha R.The role of tissue inhibitors of metalloproteinases in tumorigenesis and metastasis.Crit Rev Clin Lab Sci,2008,45:291-338.
  • [11]Visse R,Nagase H.Matrix metalloproteinases and tissue inhibitors of metalloproteinases:structure,function,and biochemistry.Circ Res,2003,92:827-839.
  • [12]Troeberg L,Nagase H.Analysis of TIMP expression and activity.Methods Mol Med,2007,135:251-267.
  • [13]Xia D,Yan LN,Tong Y,et al.Construction of recombinant adenoviral vector carrying human tissue inhibitor of metalloproteinase-1 gene and its expression in vitro.Hepatobiliary Pancreat Dis Int,2005,4:259-264.
WanfangData CO.,Ltd All Rights Reserved
About WanfangData | Contact US
Healthcare Department, Fuxing Road NO.15, Haidian District Beijing, 100038 P.R.China
Tel:+86-010-58882616 Fax:+86-010-58882615 Email:yiyao@wanfangdata.com.cn