Expression of pulmonary surfactant protein A mRNA in lungs of fetal rats with maternal intrahepatic cholestasis

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Author:
SHI Yan()
QI Hong-bo(Department of Obstetrics and Gynecology,the First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China)
Journal Title:
CHINESE JOURNAL OF PERINATAL MEDICINE
Issue:
Volume 14, Issue 05, 2011
DOI:
10.3760/cma.j.issn.1007-9408.2011.05.004
Key Word:
Pregnancy complications;Cholestasis,intrahepatic;Pulmonary surfactantassociated protein A;RNA,messenger;Rats

Abstract: Objective To investigate the pulmonary morphologic changes and pulmonary surfactant protein A (SP-A) mRNA expression in fetal rats with maternal intrahepatic cholestasis of pregnancy (ICP).Methods Animal models of intrahepatic cholestasis of pregnancy were induced by 17-α-ethinylestradiol and progesterone.Histopathologic examination of the fetal lungs was performed under light and electronic microscopes.The expression of SP-A mRNA in the fetal lungs was measured by reverse transcription-polymerase chain reaction technique.Results (1) Compared with the control group,histopathologic examination showed that fetal pulmonary tissues had dilated and congested interstitial lung capillaries,thickened alveolar septum,mild focal inflammatory exudation and focal hemorrhage in alveolus.Furthermore,reduced microvilli,mitochondrion vacuolization,cytoplasm disintegration and increased lamellar body evacuation were observed in alveolar epithelial cell II in ICP group under light and electronic microscopes.(2) Expression of SP-A mRNA in fetal lungs of ICP group (0.71 + 0.10) was significantly lower than that in control group (1.00±0.27),t=3.093,P<0.05.Conclusions Hyperbileacidemia in ICP maternal rat might lead to pathological changes in fetal pulmonary tissues.Low expression of SP-A mRNA might contribute to lesions of fetal lung with ICP.

  • [1]Zecca E,De Luca D,Marras M,et al.Intrahepatic cholestasis of pregnancy and neonatal respiratory distress syndrome.Pediatrics,2006,117:1669-1672.
  • [2]林安平,卢春冬,王琳,等.1340例妊娠期肝内胆汁淤积症的回顾性分析.中华围产医学杂志,2010,13:102-106.
  • [3]贺晶,蔡淑萍.妊娠期肝内胆汁淤积症的诊治.中华围产医学杂志,2009,12:64-67.
  • [4]邵勇,姚珍薇,李红霞,等.孕鼠肝内胆汁淤积症对母鼠和胎鼠心脏的影响.临床与实验病理学杂志,2005,21:706-709.
  • [5]Kishore U,Greenhough TJ,Waters P,et al.Surfactant proteins SP-A and SP-D:structure,function and receptors.Mol Immunol,2006,43:1293-1315.
  • [6]Goto H,Ledford JG,,Mukheriee S,et al.The role of surfactant protein A in bleomycin-induced acute lung injury.Am J Respir Crit Care Med,2010,181:1336-1344.
  • [7]Günther A,Siebert C,Schmidt R,et al.Surfactant alterations in severe pneumonia,acute respiratory distress syndrome,and cardiogenic lung edema.Am J Respir Crit Care Med,1996,153:176-184.
  • [8]Matthay MA,Zemans RL.The acute respiratory distress syndrome:pathogenesis and treatment.Annu Rev Pathol,2011,6:147-163.
  • [9]Gregory TJ,Longmore WJ,Moxley MA,et al.Surfactant chemical composition and biophysical activity in acute respiratory distress syndrome.J Clin Invest,1991,88:1976-1981.
  • [10]Brackenbury AM,Malloy JL,McCaig LA,et al.Evaluation of alveolar surfactant aggregates in vitro and in vivo.Eur Respir J,2002,19:41-46.
  • [11]Hall SB,Hyde RW,Notter RH.Changes in subphase aggregates in rabbits injured by free fatty acid.Am J Respir Crit Care Med,1994,149:1099-1106.
  • [12]舒林华,薛辛东,舒林宏,等.脂多糖诱导的急性肺损伤幼鼠肺泡Ⅱ型上皮细胞超微结构研究.国际儿科学杂志,2007,34:166-168.
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