β-lactamase genotyping of multi-drug resistant Acinetobacter baumannii in a neonatal intensive care unit

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Author:
YAN Gang-feng(Department of Neonatology,Children's Hospital of Fudan University,Shanghai 201102,China)
CAO Yun(Department of Neonatology,Children's Hospital of Fudan University,Shanghai 201102,China)
QU Di()
WANG Chuan-qing()
CHEN Chao(Department of Neonatology,Children's Hospital of Fudan University,Shanghai 201102,China)
Journal Title:
CHINESE JOURNAL OF PERINATAL MEDICINE
Issue:
Volume 14, Issue 05, 2011
DOI:
10.3760/cma.j.issn.1007-9408.2011.05.001
Key Word:
Acinetobacter baumannii;Drug resistance,bacterial;beta-lactamases;Genotype;Intensive care units,neonatal

Abstract: Objective To investigate the correlation between drug resistance and β-actamase genes of multi-drug resistance Acinetobacter baumannii (MDR-AB) in neonatal intensive care unit to provide evidence for rational antibiotics administration and nosocomial infection control.Methods Twenty-six MDR-AB strains were separated and collected from clinical specimens.The minimum inhibitory concentrations of 13 antimicrobial agents were determined by agar dilution method.Genotypes of β-lactamase were detected by polymerase chain reaction.Results The resistant rates of the 26 strains to Ceftazidime,Cefoxitin,Piperacillin-tazobactam and Ciprofloxacin were 100.0%.About 80.8% to 96.2% of these strains were resistant to the other antimicrobial drugs.Among the 26 MDR-AB strains,100% (26/26) strains possessed oxa-51,77% (20/26) possessed oxa-23 gene,54% (14/26) carried arnpC gene,both oxa-23 and ampC were identified in 42% (11/26) strains,while oxa-24,oxa-58,imp-1,imp-4 and vim-2 gene were not identified.Conclusions The drug resistance of Acinetobacter baumannii is serious,oxa-23 and ampC are the major plactamase genes carried by MDR-AB in neonatal intensive care unit.

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