Effect of lipoxin A4 on interleukin-1β production of human monocytes from severe preeclamptic women and its relationship with cytoplasm free calcium concentration-an in vitro study

( views:206, downloads:0 )
Author:
WANG Jian-fang(Department of Obstetrics and Gynecology,First Affiliated Hospital of Wenzhou Medical College,Wenzhou 325000,China)
HUANG Yin-ping(Department of Obstetrics and Gynecology,First Affiliated Hospital of Wenzhou Medical College,Wenzhou 325000,China)
HUANG Yan-jun(Department of Obstetrics and Gynecology,First Affiliated Hospital of Wenzhou Medical College,Wenzhou 325000,China)
LIU Xiao-li(Department of Obstetrics and Gynecology,First Affiliated Hospital of Wenzhou Medical College,Wenzhou 325000,China)
ZHOU Jie(Department of Obstetrics and Gynecology,First Affiliated Hospital of Wenzhou Medical College,Wenzhou 325000,China)
Wang Jing(Department of Obstetrics and Gynecology,First Affiliated Hospital of Wenzhou Medical College,Wenzhou 325000,China)
Journal Title:
CHINESE JOURNAL OF PERINATAL MEDICINE
Issue:
Volume 13, Issue 03, 2010
DOI:
10.3760/cma.j.issn.1007-9408.2010.03.005
Key Word:
Lipoxins;Pre-eclampsia;Monocytes;Interleukin-lbeta;Calcium

Abstract: Objective To investigate the effect of lipoxin A4(LXA4) on interleukin-1β(IL-1β)production of monocytes in maternal peripheral blood from severe preeelampsia women and its relationship with cytosolic free calcium([Ca2+]i)concentration. Methods Peripheral venous blood was drawn from 15 women with severe preeelampsia(SPE group)and 20 normal pregnant women (control group)who were admitted to the First Affiliated Hospital of Wenzhou Medical College from October 2008 to May 2009.Monoeytes were obtained from peripheral blood with Ficolt density gradient centrifugation and then were suspended in RPMI-1640 culture supplemented with LXA4 at the final concentrations of 0,10 and 100 nmol/L,respectively.IL-1β levels in monocytes supernatant were detected by enzyme-linked immunosorbent assay.The cytoplasma[Ca2+]i of cultured monocytes and its variations affected by LXA4 were measured by laser scanning confocal microscope. Results (1) After incubation with different concentrations of LXA4(0,10,100 nmol/L)for 24 h,the levels of IL-1β in SPE group were(63.16±8.20)pg/L,(53.71±8.08)pg/L and(43.16±6.89)pg/L,respectively, indicating a significant inhibition effect on IL-1β level in a dose-dependent manner (P<0. 05). The IL-1β levels in the control group were (19.22±7.43) pg/L, (16.99±6.32) pg/L and (15. 18±5.24) pg/L, correspondingly (P>0.05). (2) Without LXA4, the [Ca2+ ]i concentrations of monocytes in the SPE group were higher than that of the control (1028.09 ± 160. 52 vs 323.61 ±87.86, P<0. 05). After treatment with 100 nmol/L LXA4, the [Ca2+ ]i concentration of monocytes significantly decreased in the SPE group (409.67± 116.73, P<0. 05). Conclusions In vitro LXA4 may inhibit the IL-1β production in monoeytes of SPE patients through decrease of the cytoplama [Ca2+ ]i concentration.

  • [1]倪锈,黄艳君,吴敏,等.脂氧素对子痢前期患者巨噬细胞增殖和分泌功能的影响.中华妇产科杂志,2008,43:325-328.
  • [2]Liu S,Wu P,Ye D,et al.Effects of lipoxin A4 on CoCl2-induced angiogenesis and its possible mechanisms in human umbilical vein endothelial cells.Pharmacology,2009,84:17-23.
  • [3]刘松君,李咏生,周晓燕,等.脂氧素A4对缺氧损伤的人脐静脉内皮细胞的保护作用.中华妇产科杂志,2009,44:281-284.
  • [4]乐杰.妇产科学.7版.北京:人民卫生出版社,2008:92-97.
  • [5]Sibai B,Dekker G,Kupferminc M.Preeclampsia.Lancet,2005,365:785-799.
  • [6]Vizi ES,Szelenyi J,Selmeczy ZS,et al.Enhanced tumor necrosis factor specific and decreased interleukin-10 specific immune responses to LPS during the third trimester of pregnancy in mice.J Endocrinol,2001,171:355-361.
  • [7]Luppi P,Deloia JA.Monocytes of preeclamptic women spontaneously synthesize pro-inflammatory cytokines.Clin Immunol,2006,118:268-275.
  • [8]Brough D,Le Feuvre RA,Wheeler RD,et al.Ca2+Stores and Ca2+ entry differentially contribute tO the release of IL-1 beta and IL-1 alpha from murine macrophages.J Immunol,2003,170:3029-3036.
  • [9]Wilson L,Butcher CJ,Kellie S.Calcium ionophore A23187 induces interleukin-8 gene expression and protein secretion in human monocytic cells.FEBS Lett,1993,325:295-298.
  • [10]Yamamoto S,Shimizu S,Kiyonaka S,et al.TRPM2-mediated Ca2+ influx induces chemokine production in monocytes that aggravates inflammatory nemrophil infiltration.Nat Med,2008,14:738-747.
  • [11]von Dadelszen P,Wilkins T,Redman CW,et al.Maternal peripheral blood leukocytes in normal and pre-eclamptic pregnancies.BJOG,1999,105:576-581.
  • [12]Serhan CN.Lipoxins and aspirin-triggered 15-epi-lipoxin biosynthesis:an update and role in anti-inflammation and pro-resolution.Prostaglandins Other Lipid Mediat,2002,68-69:433-455.
  • [13]熊维,李天,张临洪.等.脂氧素A4通过抑制Jurkat T细胞钙池操作的钙通道电流而抑制其激活和增殖.中华微生物学和免疫学杂志,2007,27:1081-1086.
WanfangData CO.,Ltd All Rights Reserved
About WanfangData | Contact US
Healthcare Department, Fuxing Road NO.15, Haidian District Beijing, 100038 P.R.China
Tel:+86-010-58882616 Fax:+86-010-58882615 Email:yiyao@wanfangdata.com.cn