Effects of prolonged oxygen exposure at different concentrations on expressions of vascular endothelial growth factor and its receptors mRNA in pulmonary tissues of neonatal rat

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Author:
FENG Hai-yan(Department of Neonatology Children's Hospital of Fudan University, Shanghai 200032, China)
CHEN Chao(Department of Neonatology Children's Hospital of Fudan University, Shanghai 200032, China)
Journal Title:
CHINESE JOURNAL OF PERINATAL MEDICINE
Issue:
Volume 12, Issue 03, 2009
DOI:
10.3760/cma.j.issn.1007-9408.2009.03.004
Key Word:
Vascular endothelial growth factor As Vascular endothelial growth factor receptor-1; Vascular endothelial growth factor receptor-2; Oxygen inhalation therapy; Oxygen; Rats

Abstract: Objective To study the effects of prolonged oxygen exposure at different concentrations on expressions of vascular endothelial growth factor (VEGF) and its receptors (VEGF receptor-1 ,VEGFR1 and VEGF receptor-2, VEGFR2)mRNA in lungs of neonatal rat. Methods Thirty-two SD rat pups were randomly divided into control and experimental group. Rats in the experimental group were further divided into three subgroups: 30% O2, 50% O2 and 75% O2 group. Each group had 8 rats. The rats were ,sacrificed at postnatal day 21 and their right lower lobes were collected. Expressions of VEGF, VEGFR1 and VEGFR2 mRNA were detected by RT-PCR. Results There was no effect on VEGF, VEGFR1 or VEGFR2 mRNA expression in 30% O2 group. The expressions of VEGF mRNA in the 75% O2 group was 0. 48 times of the control (P<0. 05). The expressions of VEGFR1 mRNA(P<0.01) and VEGFR2 mRNA (P<0.01) in 50% O2 and 75% O2 group were lower than those of control. Condusions Prolonged exposure to moderate or high oxygen concentration may reduce the expression of VEGF and its receptors' mRNA in the lung tissues of neonatal rats.

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