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Analysis of differential protein mass spectrogram in the amniotic fluid of pre-eclampsia and normal pregnancy
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF PERINATAL MEDICINE
- Issue:
- 5
- DOI:
- 10.3760/cma.j.issn.1007-9408.2007.05.002
- Key Word:
- 先兆子(癎);妊娠;羊水;光谱法,质量,基质辅助激光解吸电离;蛋白质类
Abstract： 目的 探讨并初步筛选子(癎)前期患者的特异性羊水标志物.方法 利用CMlO蛋白芯片和表面增强激光解析离子化飞行时间质谱(SELDI TOF MS)技术,检测8例重度子(癎)前期患者(研究组)和8例晚孕期正常孕妇(对照组)羊水中差异蛋白质的相对含量.并在8例中孕期(孕20±周)正常孕妇(中孕组)羊水中寻找有无这些差异蛋白的存在及其含量变化情况. 结果 (1)研究组与对照组羊水蛋白质在相对分子质量/电荷比值(质/荷比)2000～50 000范围内,有4种不同质荷比蛋白质的相对含量有明显差异.(2)研究组质荷比为4653的蛋白质相对含量[(1.52±0.71)%]明显低于对照组[(3.16±1.64)%](P＜0.05),而质荷比为384.1、8520、8579的蛋白质相对含量分别为(7.02±4.37)%、(3.74±1.96)%、(5.09±1.93)%,均高于对照组[(2.58±1.09)%、(1.83±0.67)%、(3.06±1.57)%,P均＜0.05].(3)中孕组羊水中可以找到这四种差异蛋白质.结论 子(癎)前期患者与正常孕妇羊水蛋白质质谱存在明显差异.有4种不同质荷比相对应的蛋白质可能是子(癎)前期患者羊水特异性的生物标志物.
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