Detection of paraoxonase gene polymorphism and activity in preeclampsia
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF PERINATAL MEDICINE
- Issue:
- 1
- DOI:
- 10.3760/cma.j.issn.1007-9408.2007.01.006
- Key Word:
- 先兆子(癎)芳基二烷基磷酸酶;多态现象(遗传学)
Abstract： 目的 研究对氧磷酶1(paraoxonase 1,PON1)基因R192Q位点多态性及PON1活力和血脂脂蛋白水平在子(癎)前期(preeclampsia,PE)发病中的相关性.方法 共139例孕妇,其中PE组73例,包括轻度子(癎)前期(mild preeclampsia, MPE)34例,重度子(癎)前期(severe preeclampsia, SPE)39例,对照组为66例健康孕妇.应用全自动生化分析仪测定血脂脂蛋白,乙酸苯酯法测定PON1活力,聚合酶链反应(polymerase chain reaction, PCR)、限制性片段长度多态性(restriction fragment length polymorphism, RFLP)方法分析PON1 R192Q位点多态性.结果 (1)SPE组胆固醇(cholesterol,CHOL)、甘油三脂(triglyeride,TG)、低密度脂蛋白(low density lipoprotein, LDL)和载脂蛋白B(apolipoproteinB, apoB)均比对照组高(P＜0.05),而高密度脂蛋白(high density lipoprotein, HDL)和载脂蛋白A (apolipoprotein A, apoA)比对照组低(P＜0.05).MPE组TG、HDL、apoA和apoB与对照组差异显著(P＜0.05),CHOL和LDL与对照组差异无显著性(P＞0.05),与SPE组差异显著(P＜0.05).(2)PE组PON1活力明显低于对照组(P＜0.05).MPE组与SPE组比较差异无显著性(P＞0.05).(3)PE组和对照组间的基因型变异频率和R、Q等位基因频率均有统计学差异(P均＜0.05).(4)PON1活力比较,QQ基因型分别高于RR和RQ基因型(P均＜0.05),RQ基因型比RR基因型高(P＜0.05).结论 PE患者PON1活性下降.PON1基因R192Q位点多态性与PON1活力有关,QQ基因型PON1活力最高,其次是RQ和RR基因型.PON1 R192Q位点多态性与PE发病有关,R等位基因可能是其易感基因.
- [1]Zakaria, Ahmed,Saeid, Babaei,Graham F, Maguire,etc.Paraoxonase-1 reduces monocyte chemotaxis and adhesion to endothelial cells due to oxidation of palmitoyl, linoleoyl glycerophosphorylcholine.[J].2003,57(1).
- [2]乐杰.妇产科学[M].2004.
- [3]C E, Furlong,R J, Richter,S L, Seidel,etc.Role of genetic polymorphism of human plasma paraoxonase/arylesterase in hydrolysis of the insecticide metabolites chlorpyrifos oxon and paraoxon.[J].1988,43.
- [4]Shinohara K,Ikenoue N,Fukaya T,etc.Lipoprotein particles in preeclampsia: susceptibility to oxidative modification.[J].2000,96(1).
- [5]D, Ombres,G, Pannitteri,A, Montali,etc.The gln-Arg192 polymorphism of human paraoxonase gene is not associated with coronary artery disease in italian patients.[J].1998,18.
- [6]Odawara Masato,Tachi Yoichi,Yamashita Kamejiro.Paraoxonase Polymorphism (Gln192-Arg) Is Associated with Coronary Heart Disease in Japanese Noninsulin-Dependent Diabetes Mellitus[J].1997.
- [7]H, Uzun,A, Benian,R, Madazli,etc.Circulating oxidized low-density lipoprotein and paraoxonase activity in preeclampsia.[J].2005,60(4).
- [8]van Tol A,Katan MB,Schouten EG,etc.Replacement of dietary saturated fat with trans fat reduces serum paraoxonase activity in healthy men and women.[J].2002,51(12).
- [9]Durrington PN,Mackness MI,Mackness B.Paraoxonase and atherosclerosis.[J].2001,21(4).
- [10]Kopprasch S,Pietzsch J,Kuhlisch E,etc.Lack of Association between Serum Paraoxonase 1 Activities and Increased Oxidized Low-Density Lipoprotein Levels in Impaired Glucose Tolerance and Newly Diagnosed Diabetes Mellitus.[J].2003,88(4).
- [11]Selahattin, Kumru,Suleyman, Aydin,M Ferit, Gursu,etc.Changes of serum paraoxonase (an HDL-cholesterol-associated lipophilic antioxidant) and arylesterase activities in severe preeclamptic women.[J].2004,114.