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Effects of pigment epithelium-derived factor on neovascularization in oxygen-induced retinopathy
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF PERINATAL MEDICINE
- Issue:
- 6
- DOI:
- 10.3760/cma.j.issn.1007-9408.2006.06.007
- Key Word:
- 眼蛋白质类;神经生长因子;舍平类;视网膜病,早产儿;视网膜新生血管化
Abstract： 目的 探讨色素上皮衍生因子(pigment epithelium-derived factor,PEDF)对高氧诱导的新生小鼠视网膜病变新生血管的抑制作用.方法 选取7日龄C57BL/6J新生小鼠吸75%高氧5 d后返回空气制备早产儿视网膜病(retinopathy of prematunty,ROP)的小鼠模型.通过对模型组(n=26)和模型对照组(n=26)小鼠视网膜比较,观察吸氧对视网膜血管的影响.治疗组(n=112):采用不同剂量和注药次数给ROP小鼠右眼玻璃体内注入PEDF,左眼作为自身对照,在生后17 d各组取材做视网膜铺片和切片,对视网膜新生血管进行形态学观察和定量研究.结果 ROP小鼠在生后第12天和14天右眼注入两次PEDF后,第17天时计数突破视网膜内界膜的细胞核数,1μg组左右眼细胞核数分别是(39.43±3.28)个和(13.51±2.05)个(P=0.000);2μg组左右眼细胞核数分别是(48.40±6.24)个和(11.80±1.74)个(P=0.000).血管形态和定量结果均显示眼内注射PEDF后新生血管形成明显减少.以2μg作为注射PEDF剂量,单次注药无效,二次和三次眼内注药有效果.结论 玻璃体内注入PEDF能有效抑制视网膜新生血管的生长,同时不影响正常血管的发育,可能成为防治ROP有效的血管抑制因子.
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