You Position: Home > Paper
Study on Apoptosis and Expression of Fas, FasL in Placenta of Intrahepatic Cholestasis of Pregnancy
( views:287, downloads:16 )
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF PERINATAL MEDICINE
- Issue:
- 4
- DOI:
- 10.3760/cma.j.issn.1007-9408.2003.04.002
- Key Word:
- 妊娠并发症;胆汁淤积,肝内;胎盘;细胞凋亡;抗原,CD95;膜糖蛋白类
Abstract： 目的探讨妊娠肝内胆汁淤积症(intrahepatic cholestasis of pregnancy, ICP)胎盘组织Fas及FasL (Fasligand)表达在细胞凋亡中的作用. 方法采用dUTP缺口末端标记法(TUNEL)和免疫组化技术对31例ICP患者胎盘组织中的细胞凋亡、Fas、FasL的基因表达进行检测,并以正常妊娠胎盘组织31例作为对照组. 结果 ICP组胎盘组织中细胞滋养细胞、合体滋养细胞、蜕膜细胞及绒毛间质细胞的凋亡指数(AI)分别为49.09±9.13 (t=13.41)、46.64±9.77 (t=12.16)、35.09±9.49 (t=8.43)、38.74±9.70 (t=11.28),明显高于对照组(P<0.005).ICP组细胞滋养细胞和合体滋养细胞Fas表达明显增强(P<0.005),FasL在细胞滋养细胞、合体滋养细胞、蜕膜细胞及绒毛间质细胞中的表达明显低于对照组(P<0.005). 结论 ICP患者胎盘组织中Fas表达增强,FasL表达减弱,可导致母胎间免疫耐受的破坏,胎盘细胞凋亡增加,可能与ICP患者胎盘功能减退有关.
- [1]Sharma S,Balkundi DR,Dougherty J,etc.Labor-associated changes in Fas ligand expression and function in human placenta.[J].2000,47(3).
- [2]O M, Alsulyman,J G, Ouzounian,M, Ames-Castro,etc.Intrahepatic cholestasis of pregnancy: perinatal outcome associated with expectant management.[J].1996,175.
- [3]Bamberger A.-M..Epression of the Apoptosis-Inducing FAS Ligand (FASL) in Human First and Third Trimester Placenta and Choriocarcinoma Cells[J].1997.
- [4]T S, Griffith,T, Brunner,S M, Fletcher,etc.Fas ligand-induced apoptosis as a mechanism of immune privilege.[J].1995,270.
- [5]R, Axt,A C, Kordina,R, Meyberg,etc.Immunohistochemical evaluation of apoptosis in placentae from normal and intrauterine growth-restricted pregnancies.[J].1999,26.
- [6]Hetts SW.To die or not to die: an overview of apoptosis and its role in disease (see comments)[J].1998,279(4).
- [7]Kokawa K,Shikone T,Nakano R.Apoptosis in the human uterine endometrium during the menstrual cycle.[J].1996.
- [8]D.M. Nelson.Apoptotic changes occur in syncytiotrophoblast of human placental villi where fibrin type fibrinoid is deposited at discontinuities in the villous trophoblast[J].1996,17(7).