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Number of Fetal Nucleated Red Blood Cell in Maternal Blood and Placenta in Pregnancies with Intrauterine Growth Retardation
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF PERINATAL MEDICINE
- Issue:
- 2
- DOI:
- 10.3760/cma.j.issn.1007-9408.2002.02.003
- Key Word:
- 妊娠;幼红细胞;胎盘;胎儿生长迟缓
Abstract： 目的探讨孕妇外周血及胎盘组织中胎儿有核红细胞(nucleated red blood cell, NRBC)的出现频率与胎儿生长受限(fetal growth restriction, FGR)的关系. 方法对20例孕28～36周,年龄21～30岁(包括9例FGR)的孕妇外周血进行不连续密度梯度离心,对分离后的细胞进行制片,显微镜下进行NRBC计数,比较组间差异;追随至终止妊娠时,对胎盘组织进行切片,显微镜下进行NRBC计数,比较组间差异;显微操作法获取5例单个NRBC行引物延伸预扩增(PEP)和聚合酶链反应(PCR),验证其胎儿细胞来源. 结果 9例FGR妊娠妇女外周血中NRBC数目从12个/7 ml～40个/7 ml不等,平均为22.6个/7 ml.而同孕龄正常妊娠妇女外周血中NRBC数目从0个/7 ml～10个/7 ml不等,平均为5.4个/7 ml,两者间差异有极显著性(P<0.001);FGR妊娠妇女胎盘绒毛间质血管中NRBC数目从2个/20HP～5个/20HP不等,平均为2.8个/20HP.而正常妊娠妇女则从0个/20HP～2个/20HP不等,平均为0.6个/20HP,两者间差异有显著性(P<0.05). 结论 FGR妊娠妇女其外周血与胎盘组织中NRBC数目明显升高.
- [1]邹丽,朱剑文.孕妇外周血中胎儿有核红细胞的出现频率及其与孕周的关系[J].2000,(4).
- [2]J L, Simpson,S, Elias.Isolating fetal cells from maternal blood. Advances in prenatal diagnosis through molecular technology.[J].1993,270.
- [3]叶晓静,邹丽.从孕妇外周血中分离胎儿有核红细胞方法学初探[J].1999,(4).
- [4]F. von Eggeling,Susanne Michel,Michael Günther,etc.Determination of the origin of single nucleated cells in maternal circulation by means of random PCR and a set of length polymorphisms[J].1997,99(2).
- [5]Ikawa K,Ukita T,Takabayashi H,etc.Development of non-invasive fetal DNA diagnosis from maternal blood.[J].1995,15(1).
- [6]R, Al-Mufti,C, Lees,G, Albaiges,etc.Fetal cells in maternal blood of pregnancies with severe fetal growth restriction.[J].2000,15.
- [7]Van-Ommen GJ,Beverstock GC,Tanke HJ,etc.Prenatal diagnosis of trisomy 13 on fetal cells obtained from maternal blood after minor enrichment.[J].1998,18(10).
- [8]Cui K.-H.,Haan E. A,Wang L.-J.,etc.Optimal polymerase chain reaction amplification for preimplantation diagnosis in cystic fibrosis ((Delta)F508)[J].1995.
- [9]L M, Korst,J P, Phelan,M O, Ahn,etc.Nucleated red blood cells: an update on the marker for fetal asphyxia.[J].1996,175.
- [10]Yun-Ling Zheng,Maryann Demaria,Dongkai Zhen,etc.Flow sorting of fetal erythroblasts using intracytoplasmic anti-fetal haemoglobin: Preliminary observations on maternal samples[J].1995,15(10).