Abstract： Objective To investigate the distribution of hepatitis B virus (HBV) genotypes in Qingdao,and the relationship of HBV genotypes with the serum HBV-DNA levels and HBV YMDD spontaneous mutation of patients,then to discuss the clinical significance.Methods Hepatitis B virus genotypes and YMDD spontaneous mutation of 144 patients were detected by real time PCR (Taqman probe),then the results were analyzed by statistical method.Results Of the 144 patients,130 (90.3％ )were genotype C,12 (8.3％) were genotype B,and 2 (1.4％) were neither genotype B nor genotype C;33 (22.9％) were detected to have YMDD mutation,and 25 (75.5％) were YVDD positive,3 (9.1％ )were YIDD positive,5 ( 15.2％ ) were YVDD and YIDD positive.There were no significant differences between clinical diagnosis,serum HBV-DNA levels,YMDD spontaneous mutation and HBV genotypes (P ＞0.05 ).Conclusion Genotype C is the dominant position for HBV genotype in Qingdao.Untreated patients with chronic hepatitis B have YMDD spontaneous mutation.HBV genotypes have no association with YMDD spontaneous mutation and the development of diseases.