Expression of interleukin -32 in glioma and its regulatory mechenism

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Author:
MA Zhi-yuan(Department of Neurology, Second People's Hospital of Zhanjiang, Zhanjiang 524003, China)
GUO You-feng()
CHEN Yan(Department of Neurology, Second People's Hospital of Zhanjiang, Zhanjiang 524003, China)
GUAN Chun-hong(Department of Neurology, Second People's Hospital of Zhanjiang, Zhanjiang 524003, China)
HUANG Shu-ming(Department of Neurology, Second People's Hospital of Zhanjiang, Zhanjiang 524003, China)
Journal Title:
Chinese Journal of Neuromedicine
Issue:
Volume 11, Issue 09, 2012
DOI:
10.3760/cma.j.issn.1671-8925.2012.09.002
Key Word:
Glioma;Interleukin 32;Expression

Abstract: Objective To discuss the expression of interleukin-32 (IL-32) in glioma and its regulatory mechanism. Methods RT-PCR and Western blotting were used to detect the mRNA and protein expression levels of IL-32 in CHG-5 and U251 cell lines in vitro cultured for 3 d.And then, 10 ng/mL IL-1β, IL-4, IL-6, IL-10, IL-17, tumour necrosis factor alpha (TNF-α), TNF-β and interferon-gamma(IFN-γ) were put into U251 cell lines for 24 h,and RT-PCR was employed to detect the mRNA expression of IL-32; meanwhile,RT-PCR was also employed to detect the mRNA expression of IL-32 after the treatment of IL- 1 β,TNF-α and IFN-γ for different times,respectively. Results The mRNA expression of IL-32 in U251 cells was (6.41±1.12)-fold higher than that in CHG-5 cells (P<0.05);the protein expression level of IL-32 in U251 cells (0.95±0.42) was significantly higher than that in CHG-5 (0.28±0.13,P<0.05).IL-1β,TNF-α and IFN-γ markedly enhanced the IL-32 mRNA expression at 24 h after treatment as compared with that before treatment, and dose- and time-dependent manners of IL-32 mRNA expression were noted (P<0.05). Conclusion High expression of IL-32 is noted in glioma; IL-1β, TNF-α and IFN-γ can regulate the IL-32 mRNA expression with dose- and time-dependent manners.

  • [1]段纪俊,陈万青,张思维,等.中国恶性肿瘤死亡率的国际比较[J].中国社会医学杂志,2009,26(6):377-378.
  • [2]梁丽琴,金刚,党建章,等.儿茶素对脑胶质瘤细胞U251增殖的抑制作用及机制初探[J].实用医学杂志,2011,27(9):1526-1528.
  • [3]Karin M,Greten FR.NF-kappaB:linking inflammation and immunity to cancer development and progression[J]. Nat Rev Immunol,2005,5(10):749-759.
  • [4]Jee SH,Shen SC,Chiu HC,et al.Over-expression of interleukin-6 in human basal cell carcinoma cell lines increases anti-apoptotic activity and tumorigenic potency[J]. Oncogene.?2001,20 (2):198-208.
  • [5]Cacev T,Radosevic S,Krizanac S,et al.Infuence of interleukin-8 and interleukin-10 on sporadic colon cancer development and progression[J].Carcinogenesis,2008,29(8):1572-1580.
  • [6]Carlo S,Emma D.Expression of IL-32 in Human Lung Cancer Is Related to the Histotype and Metastatic Phenotype[J].Am J Res Crit Care Med,2009,180(12):769-779.
  • [7]Atsushi N,Akira A,Osamu I,et al.Interleukin-32 Expression in the Pancreas[J].J Biol Chem,2009,284(26):17868-17876.
  • [8]JH Oh,MC Cho,JH Kim,et al.IL-32γinhibits cancer cell growth through inactivation of NF-κB and STAT3 signals[J].Oncogene,2011,28(30):54-59.
  • [9]Anako KP.Charles.Molecular characterization of IL-32 in human endothelial cells[J].Cytokine,2009,46(3):351-358.
  • [10]Clevers H.At the crossroads of inflammation and cancer[J].Cell,2004,118(6):671-674.
  • [11]Hussain SP.Inflammation and cancer:an ancient link with novel potentials[J].Cancer,2007,121(11):2373-2380.
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