Expressions of Drebrins and lcam-5 in mouse cerebral cortex with Fmr-1 gene knockout and their significance in fragile X syndrome

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Author:
XV Qin(Department of Children's Health Care, Children's Hospital, School of Medicine of Zhejiang University, Hangzhou 310003, China)
ZHU Zhi-wei(Department of Children's Health Care, Children's Hospital, School of Medicine of Zhejiang University, Hangzhou 310003, China)
ZHAO Zheng-yan(Department of Children's Health Care, Children's Hospital, School of Medicine of Zhejiang University, Hangzhou 310003, China)
Journal Title:
Chinese Journal of Neuromedicine
Issue:
Volume , Issue 07, 2012
DOI:
10.3760/cma.j.issn.1671-8925.2012.07.003
Key Word:
Fragile X Syndrome;Dendritic spine;Drebrins;lcam-5

Abstract: [Objective]To investigate and compare the changes of Drebrin A,Drebrin E and lcam-5 mRNA levels in the cerebral cortex of Frr-1 gene knockout mouse during brain development periods.[Methods]Fmr-1 gene knockout (KO) male mice and their wild type (WT) counterparts were chosen in our experiment (4≤n≤ 10);the levels of target mRNAs were detected by real time quantitative PCR;check points were set on the 7th,14th,21th and 28rh postnatal d.[Results] The mRNA level of Drebrin A in the KO group was significantly lower than that in the WT group on the 14th postnatal d,while that of Drebrin E was significantly higher than that in the WT group (P<0.05).The mRNA level of lcam-5 in the KO group was significantly higher than that in the WT group on the 14th and 21th postnatal d (P<0.05).[Conclusion] The delayed shift of Drebrin A to Drebrin E and transitional over-expression of lcam-5 in developmental cerebral cortex are the reasons for mental retardation in Fragile X Syndrome.

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