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Adenovirus-mediated glucose transporter 3 infection in PC12 cells of rats after hypoxiareperfusion injury

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Author:
No author available
Journal Title:
Chinese Journal of Neuromedicine
Issue:
7
DOI:
10.3760/cma.j.issn.1671-8925.2012.07.002
Key Word:
缺氧再灌注损伤;葡萄糖转运体3;葡萄糖摄取率;细胞凋亡;核因子-kB;Hypoxia and reperfusion injury;Glucose transporter 3;Glucose uptake rate;Apoptosis;Nuclear factor-kB

Abstract: [Objective]To investigate the effect of adenovirus-mediated glucose transporter 3 (Ad-GLUT3) infection on PC 12 cells of rats after hypoxia-reperfusion injury.[Methods]PC 12 cells treated with hypoxia and re--oxygenation were prepared;after PC12 cells being infected by Ad-GLUT3 (experimental group),adenovirus encoding green fluorescent protein (Ad-GFP group) and blank plasmid (control group),respectively,flow cytometry (FC) and TUNEL assay were used to analyze the apoptosis of PC12 cells;the glucose uptake rate was detected by isotopic-scintiscan,and the expression of nuclear factor (NF-kB) was detected by Western blotting.[Results] FC indicated that the apoptotic rate in the experimental group (4.7%±1.75%) was significantly lower as compared with that in the Ad-GFP group (14.25%±3.46%) and control group (13.1%±2.98%,P<0.05);TUNEL assay showed that the apoptotic rate in the experimental group (7.7%±3.31%) was significantly lower as compared with that in the Ad-GFP group (17.1%±3.10%) and control group (15.2%±2.70%,P<0.05).And the glucose uptake rate in the experimental group (603%±13.1%) was obviously higher as compared with that in the Ad-GFP group (300%±8.77%) and control group (319%±11.2%,P<0.05);and the NF-k<B level in the experimental group (0.92±0.17) was significantly lower as compared with that in the Ad-GFP group (1.02±0.24) and control group (1.06±0.21,P<0.05).[Conclusion] After being infected by Ad-GLUT3,the PC12 cells treated with hypoxia and re-oxygenation enjoy elevated glucose uptake rate and inhibited apoptotic rate.

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