Distribution and expression changes of tight junctional protein JAM-1 in rat models after intracerebral hemorrhage

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Author:
JIANG Wei-ping(Department of Neurosurgery,Guangdong Neurosurgery Institute and Key Laboratory on Brain Function Repair and Regeneration,Zhujiang Hospital, Southern Medical University, Guungzhou 510282, China)
CHEN Yi-zhao(Department of Neurosurgery,Guangdong Neurosurgery Institute and Key Laboratory on Brain Function Repair and Regeneration,Zhujiang Hospital, Southern Medical University, Guungzhou 510282, China)
LI Bing(Department of Neurosurgery,Guangdong Neurosurgery Institute and Key Laboratory on Brain Function Repair and Regeneration,Zhujiang Hospital, Southern Medical University, Guungzhou 510282, China)
YANG Shuo(Department of Neurosurgery,Guangdong Neurosurgery Institute and Key Laboratory on Brain Function Repair and Regeneration,Zhujiang Hospital, Southern Medical University, Guungzhou 510282, China)
DENG Xin-qing(Department of Neurosurgery,Guangdong Neurosurgery Institute and Key Laboratory on Brain Function Repair and Regeneration,Zhujiang Hospital, Southern Medical University, Guungzhou 510282, China)
FU Zheng-hao(Department of Neurosurgery,Guangdong Neurosurgery Institute and Key Laboratory on Brain Function Repair and Regeneration,Zhujiang Hospital, Southern Medical University, Guungzhou 510282, China)
DU Mou-xuan(Department of Neurosurgery,Guangdong Neurosurgery Institute and Key Laboratory on Brain Function Repair and Regeneration,Zhujiang Hospital, Southern Medical University, Guungzhou 510282, China)
TANG Yan-ping(Department of Neurosurgery,Guangdong Neurosurgery Institute and Key Laboratory on Brain Function Repair and Regeneration,Zhujiang Hospital, Southern Medical University, Guungzhou 510282, China)
KE Yi-quan(Department of Neurosurgery,Guangdong Neurosurgery Institute and Key Laboratory on Brain Function Repair and Regeneration,Zhujiang Hospital, Southern Medical University, Guungzhou 510282, China)
Journal Title:
Chinese Journal of Neuromedicine
Issue:
Volume , Issue 07, 2012
DOI:
10.3760/cma.j.issn.1671-8925.2012.07.001
Key Word:
JAM-1;Intracerebralhemorrhage;Tight junction;Blood-brain barrier;Inflammation

Abstract: [Objective]To explore the distribution and expression changes of tight junctional protein JAM-1 in rat models after intracerebral hemorrhage (ICH) and their significance.[Methods]One hundred and twenty-eighty healthy male SD rats were randomly divided into normal control group (n=16) and ICH group (n=112),and the ICH models were induced by stereotactically injecting 75 uL autologous blood into the right caudate nucleus.Seven time points after ICH (6,12,24 and 48 h,and 3,7 and 14 d after ICH,16 rats for each time point) were chosen.BBB permeability was evaluated by Evans blue dye extravasation.The distribution and expression of JAM-1 were detected by immunofluorescence and real-time quantitative PCR.[Results] As compared with that in the normal control group,BBB permeability in the ICH group significantly increased at 24 and 48 h,and 3 and 7 d after ICH (P<0.05).JAM-1 expression decreased at blood vessels at 12,24 and 48 h after ICH,and JAM-1 expressed at the circulatingleukocytes3 dafterlCH,and abundant JAM-1 positive cells around hematoma were noted in the ED-l-positve macrophages 7 d after ICH.JAM-I mRNA significantly decreased at 12,24 and 48 h after ICH,and significantly increased 7 d after ICH as compared with that in the normal control group (P<0.05).[Conclusion] JAM-1 experssion changes not only participate in regulation of BBB permeability but also play roles in inflammatory insult after ICH.

  • [1]Wang J.Preclinical and clinical research on inflammation after intracerebral hemorrhage[J].Prog Neurobiol,2010,92(4):463-477.
  • [2]Keep RF,Xiang J,Ennis SR et al.Blood-brain barrier function in intracerebral hemorrhage[J].Acta Neurochir Suppl,2008,105:73-77.
  • [3]Cardoso FL,Brites D,Brito MA.Looking at the blood-brain barrier,molecular anatomy and possible investigation approaches[J].Brain Res Rev,2010,64(2):328-363.
  • [4]Wojcikiewicz EP,Koenen RR,Fraemohs L,et al.LFA-1 binding destabilizes the JAM-A homophilic interaction during leukocyte transmigration[J].Biophys J,2009,96(I):285-293.
  • [5]Laukoetter MG,Nava P,Lee WY,et al.JAM-A regulates permeability and inflammation in the intestine in vivo[J].J Exp Med,2007,204(13):3067-3076.
  • [6]Kellner CP,Connolly ES Jr.Neuroprotective strategies for intracerebral hemorrhage:trials and translation[J].Stroke,2010,4 1(10 Suppl):S99-S102.
  • [7]Gebel JM Jr,Jauch EC,Brott TG,et al.Natural history of porihernatomal edema in patients with hyperacute spontaneous intracerebral hemorrhage[J].Stroke,2002,33(1 1):2631-2635.
  • [8]李兵,陈祎招,蒋伟平,等.脑出血后血脑屏障紧密连接蛋白occludin的表达变化[J].中华神经医学杂志,2012,11(6):575-580.
  • [9]Severson EA,Parkos CA.Mechanisms ofoutside-in signaling at the tight junction by junctional adhesion molecule A[J].Ann N Y Acad Sci,2009,1165:10-18.
  • [10]Laukoetter MG,Nava P,Lee WY,et al.JAM-A regulates permeability and inflammation in the intestine in vivo[J].J Exp Med,2007,204(13):3067-3076.
  • [11]Wojcikiewicz EP,Koeoen RR Fraemohs L,et al.LFA-1 binding destabilizes the JAM-A homophilic interaction during leukocyte transmigration[J].Biophys J,2009,96(1):285-293.
  • [12]Nourshargh S,Krombach F,Dejana E.The role of JAM-A and PECAM-1 in modulating leukocyte infiltration in inflamed and ischemc tissues[J].J Leukoc Biol,2006,80(4):714-718.
  • [13]Weber C,Fraemohs L,Dejana E.The role of junctional adhesion molecules in vescular inflammation[J].Nat Rev lmnunol,2007,7(6):467-477.
  • [14]Wang J,Dore S.Inflammation after intracerebral hemorrhage[J].J Cereb Blood Flow Metal,2007,27(5):894-908.
  • [15]Pérez de ia Ossa N,Sobrino T,Silva Y,et al.Iron-related brain darnage in patients with intracerebral hemorrhage[J].Stroke,2010,41(4):810-813.
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